Decamethonium Bromide
Based on 1 publication(s) in Google Scholar
Decamethonium Bromide is an acetylcholine receptor inhibitor and muscle relaxant. Decamethonium Bromide first induces depolarization of skeletal muscles, and then binds to postsynaptic acetylcholine receptors to induce persistent paralysis.\n
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- Purity: 98.66%
- CAS No.: 541-22-0
- 화학식: C16H38Br2N2
- 분자량:418.29
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보관:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Decamethonium Bromide
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| J774.1 | IC50 |
>100 μM
Compound: 3h
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Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining
Cytotoxicity against mouse J774.1 cells after 48 hrs by alamar blue satining
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[PMID: 20382538] |
Decamethonium bromide (11.9-1190 μM; 48 h after 24 h of differentiation induction) does not inhibit growth or survival of C2C12 skeletal muscle cells at concentrations up to 1190 μM, but inhibits MHC expression in a concentration-dependent manner during early differentiation, with dramatic effects observed at ≥119 μM[1].
Decamethonium bromide (11.9-478 μM; 48 h after 7 days of differentiation induction) inhibits MHC expression in a concentration-dependent manner in terminally differentiated C2C12 myotubes, with significant effects observed at ≥47.8 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C2C12 skeletal muscle cell line (early differentiation stage)
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Concentration:11.9 μM, 47.8 μM, 119 μM, 478 μM, 1190 μM
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Incubation Time:48 h (after 24 h of differentiation induction)
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Result:Showed no significant difference in DNA count (cell number) relative to controls at all tested concentrations.
Was unaffected relative to controls at 11.9 μM and 47.8 μM.
Dramatically inhibited MHC expression at concentrations ≥119 μM, with fewer MHC-positive cells appearing rounded rather than spindle-shaped.
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Cell Line:C2C12 skeletal muscle cell line (late differentiation stage)
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Concentration:11.9 μM, 47.8 μM, 119 μM, 478 μM
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Incubation Time:48 h (after 7 days of differentiation induction)
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Result:Was similar to controls at 11.9 μM.
Inhibited MHC expression at concentrations ≥47.8 μM, with fewer MHC-positive cells appearing rounded rather than long and cylindrical.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 541-22-0
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Appearance Solid
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분자량 418.29
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화학식 C16H38Br2N2
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Color White to off-white
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SMILES
C[N+](C)(C)CCCCCCCCCC[N+](C)(C)C.[Br-].[Br-]
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618
용액&용해도
DMSO : 83.33 mg/mL (199.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (119.53 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (239.07 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (272 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Macharia R, et al. Decamethonium bromide-mediated inhibition of embryonic muscle development. Anat Embryol (Berl). 2004;208(1):75-85. [Content Brief]
[2]. COLON-YORDAN E, et al. An evaluation of the use of thiopental and decamethonium bromide for rapid endotracheal intubation. Anesthesiology. 1953 May;14(3):255-77. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.3907 mL | 11.9534 mL | 23.9069 mL | 59.7671 mL |
| 5 mM | 0.4781 mL | 2.3907 mL | 4.7814 mL | 11.9534 mL | |
| 10 mM | 0.2391 mL | 1.1953 mL | 2.3907 mL | 5.9767 mL | |
| 15 mM | 0.1594 mL | 0.7969 mL | 1.5938 mL | 3.9845 mL | |
| 20 mM | 0.1195 mL | 0.5977 mL | 1.1953 mL | 2.9884 mL | |
| 25 mM | 0.0956 mL | 0.4781 mL | 0.9563 mL | 2.3907 mL | |
| 30 mM | 0.0797 mL | 0.3984 mL | 0.7969 mL | 1.9922 mL | |
| 40 mM | 0.0598 mL | 0.2988 mL | 0.5977 mL | 1.4942 mL | |
| 50 mM | 0.0478 mL | 0.2391 mL | 0.4781 mL | 1.1953 mL | |
| 60 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9961 mL | |
| 80 mM | 0.0299 mL | 0.1494 mL | 0.2988 mL | 0.7471 mL | |
| 100 mM | 0.0239 mL | 0.1195 mL | 0.2391 mL | 0.5977 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Decamethonium Bromide Related Classifications
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