Flonicamid
Based on 1 Customer Validation
Flonicamid (IKI220) is an antifeedant Insecticide active against Hemiptera, Thysanoptera, and piercing-sucking pests including aphids of all species, developmental stages and morphs. Flonicamid inhibits the inward rectifier potassium channel NlKir1, blocking stylet penetration, salivation, sap feeding, honeydew secretion, and fluid secretion by isolated Malpighian tubules. Flonicamid reduces renal excretory function in female Culex pipiens pallens. Flonicamid rapidly disrupts insect feeding behavior without neurotoxic poisoning symptoms, induces irreversible starvation, and increases the mortality of nymph progeny of adult aphids exposed to this agent.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.71%
- CAS No.: 158062-67-0
- 화학식: C9H6F3N3O
- 분자량:229.16
-
보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- 순도&문서
- References
-
Help & FAQs
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
0.64 μM
|
Inhibition of inward K+ currents in Nilaparvata lugens NlKir1-transfected HEK293 cells assessed by whole-cell patch-clamp electrophysiology assay with 4-10 min incubation.
Inhibition of inward K+ currents in Nilaparvata lugens NlKir1-transfected HEK293 cells assessed by whole-cell patch-clamp electrophysiology assay with 4-10 min incubation.
|
29842935 |
| HEK293 | IC50 |
1.96 μM
|
Inhibition of Nilaparvata lugens NlKir1-mediated Tl+ flux in stably transfected HEK293 cells assessed by Thallium flux assay with 20 min incubation.
Inhibition of Nilaparvata lugens NlKir1-mediated Tl+ flux in stably transfected HEK293 cells assessed by Thallium flux assay with 20 min incubation.
|
29842935 |
Flonicamid (0.2-12.5 mg AI L−1; 5 days) potently kills Aphis gossypii, Rhopalosiphum padi, Schizaphis graminum, and Lipaphis erysimi aphids on their respective host plants, with LC50 values between 0.64 and 2.01 mg AI L−1 after 5 days of exposure[1].
Flonicamid (0.2-12.5 mg AI L-1; 5 days) potently kills Myzus persicae first-instar nymphs, wingless adults, and winged adults on Japanese radish leaves, with LC50 values between 0.61 and 0.77 mg AI L-1 after 5 days of exposure[1].
Flonicamid potently inhibits inward K+ currents in NlKir1-transfected HEK293 cells with an IC50 of 0.64 μM[2].
Flonicamid (0.5-20 μM; 20 min) inhibits NlKir1-mediated thallium flux in stably transfected HEK293 cells with an IC50 of 1.96 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Flonicamid exhibits nearly identical toxicity to insecticide-susceptible and resistant Nilaparvata lugens populations, with an LC50 of 44.64 mg/L for the susceptible strain and no cross-resistance to other common insecticides[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:(2-4 day-old blood-fed adult females)[2]
-
Dosage:0-57.3 ng/adult
-
Administration:topical application to abdomen; single dose
-
Result:Inhibited renal excretion in a dose-dependent manner, with an ED50 of 9.50 ng/adult.
Caused dose-dependent incapacitation (flightless or dead) at 48 h, with an LD50 of 15.38 ng/female.
Chemical Information
-
CAS No. 158062-67-0
-
Appearance Solid
-
분자량 229.16
-
화학식 C9H6F3N3O
-
Color Light brown to brown
-
SMILES
N#CCNC(C1=C(C(F)(F)F)C=CN=C1)=O
-
Synonyms
IKI220
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 100 mg/mL (436.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.91 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Morita M, et al. Flonicamid, a novel insecticide with a rapid inhibitory effect on aphid feeding. Pest management science. 2007 Oct;63(10):969-73. [Content Brief]
[2]. Ren M, et al. Block of Kir channels by flonicamid disrupts salivary and renal excretion of insect pests. Insect biochemistry and molecular biology. 2018 Aug;99:17-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3638 mL | 21.8188 mL | 43.6376 mL | 109.0941 mL |
| 5 mM | 0.8728 mL | 4.3638 mL | 8.7275 mL | 21.8188 mL | |
| 10 mM | 0.4364 mL | 2.1819 mL | 4.3638 mL | 10.9094 mL | |
| 15 mM | 0.2909 mL | 1.4546 mL | 2.9092 mL | 7.2729 mL | |
| 20 mM | 0.2182 mL | 1.0909 mL | 2.1819 mL | 5.4547 mL | |
| 25 mM | 0.1746 mL | 0.8728 mL | 1.7455 mL | 4.3638 mL | |
| 30 mM | 0.1455 mL | 0.7273 mL | 1.4546 mL | 3.6365 mL | |
| 40 mM | 0.1091 mL | 0.5455 mL | 1.0909 mL | 2.7274 mL | |
| 50 mM | 0.0873 mL | 0.4364 mL | 0.8728 mL | 2.1819 mL | |
| 60 mM | 0.0727 mL | 0.3636 mL | 0.7273 mL | 1.8182 mL | |
| 80 mM | 0.0545 mL | 0.2727 mL | 0.5455 mL | 1.3637 mL | |
| 100 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0909 mL |
Flonicamid Related Classifications
HY-119649 Related Classifications
Powered by Bioz