GL67
GL67 (N4-Spermine cholesteryl carbamate) is a cationic lipid that serves as a non-viral siRNA delivery vector and a non-viral gene transfer vector. GL67 enhances the encapsulation efficiency of siRNA in liposomal formulations and promotes the cellular uptake of siRNA across cancer cell membranes. GL67 mediates the transduction of plasmid DNA into cells. GL67 can be used in drug delivery-related research.
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- CAS No.: 179075-30-0
- 화학식: C38H70N4O2
- 분자량:614.99
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
GL67 lipid complexes display enlarged particle sizes and positively shifted zeta potentials as the cationic lipid-to-siRNA molar ratio rises from 1:1 to 3:1, with the 100% GL67 formulation at 3:1 generating a high positive zeta potential of 47 mV. It elevates siRNA encapsulation efficiency across all tested ratios, and complete encapsulation is attained with less GL67 at higher lipid-siRNA ratios[1].
GL67 pentahydrochloride-based lipid complex at a 3:1 cationic lipid/siRNA molar ratio shows favorable metabolic and membrane integrity compatibility with A549 cells and boosts cellular uptake of Cy3-siRNA in A549 cells[1].
GL67 (GL-67) mediated markedly lower gene transfection efficiency in polarized differentiated primary human and canine tracheobronchial epithelial cells cultured at the air-liquid interface, along with suppressed cell division and elevated transepithelial electrical resistance. Its transfection capacity dropped with higher cell confluence in plate-grown unpolarized human bronchial epithelial cells and declined in growth-arrested, poorly proliferative primary human bronchial epithelial cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
GL67:pDNA complexes (0.6 mM GL67 complexed with 3.6 mM pCF1-CAT; intranasal bolus (lungs); nasal perfusion) mediate >1000-fold higher gene expression than naked pDNA in the lungs of healthy BALB/c mice, but only minimal, statistically insignificant enhancement of gene expression in the nasal epithelium[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 179075-30-0
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분자량 614.99
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화학식 C38H70N4O2
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SMILES
C[C@@]12[C@]3([H])[C@](CC=C1C[C@H](CC2)OC(N(CCCN)CCCCNCCCN)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C
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Synonyms
N4-Spermine cholesteryl carbamate
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
[1]. Jarallah SJ, et al. GL67 lipid-based liposomal formulation for efficient siRNA delivery into human lung cancer cells. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society. 2023 Jul;31(7):1139-1148. [Content Brief]
[2]. Jiang C, et al. Efficiency of cationic lipid-mediated transfection of polarized and differentiated airway epithelial cells in vitro and in vivo. Human gene therapy. 1998 Jul 20;9(11):1531-42. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
GL67 Related Classifications
HY-149037 Related Classifications
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Keywords
- GL67
- 179075-30-0
- N4-Spermine cholesteryl carbamate
- GL 67
- GL-67
- Liposome
- Drug Intermediate
- airway epithelial cells
- siRNA
- chloride transport defects
- transgenic mice
- A549 cells
- cystic fibrosis
- cancer cell membranes
- lung cancer
- normal human bronchial epithelial cells
- canine tracheobronchial epithelial cells
- Inhibitor
- inhibitor
- inhibit