IOX5
Based on 1 Customer Validation
IOX5 is a selective prolyl hydroxylase (PHD) inhibitor with an IC50 of 0.19 μM for PHD2. IOX5 stabilizes HIF-1α in acute myeloid leukemia (AML) cells, inhibits cell proliferation and induces apoptosis. IOX5 has anti-leukemia activity.
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- Purity: 99.39%
- 화학식: C17H19F3N4O2
- 분자량:368.35
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
IC50: 0.19 μM (PHD2)[1]
In Vitro
IOX5 (0.5-100 μM; 24-96 h) stabilizes the expression of HIF-1α, inhibits cell proliferation and induces apoptosis in AML cell lines[1].
IOX5 (50 μM; 24-96 h) upregulates the expression of BNIP3 protein in AML cells and THP-1 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Murine iMLL-AF9 LSK cells into doxycycline(HY-N0565)-treated recipient mice[1]
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Dosage:30 mg/kg
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Administration:Intraperitoneal injection (i.p.); Twice a day; 2 weeks
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Result:Substantially increased survival of leukemic mice.
Significantly decreased LSC frequency.
Chemical Information
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Appearance Solid
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분자량 368.35
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화학식 C17H19F3N4O2
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Color White to off-white
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SMILES
FC(F)([C@H]1CC[C@H](CNC(C2=CNC(N3N=CC=C3)=CC2=O)=O)CC1)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 50 mg/mL (135.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7148 mL | 13.5740 mL | 27.1481 mL | 67.8702 mL |
| 5 mM | 0.5430 mL | 2.7148 mL | 5.4296 mL | 13.5740 mL | |
| 10 mM | 0.2715 mL | 1.3574 mL | 2.7148 mL | 6.7870 mL | |
| 15 mM | 0.1810 mL | 0.9049 mL | 1.8099 mL | 4.5247 mL | |
| 20 mM | 0.1357 mL | 0.6787 mL | 1.3574 mL | 3.3935 mL | |
| 25 mM | 0.1086 mL | 0.5430 mL | 1.0859 mL | 2.7148 mL | |
| 30 mM | 0.0905 mL | 0.4525 mL | 0.9049 mL | 2.2623 mL | |
| 40 mM | 0.0679 mL | 0.3394 mL | 0.6787 mL | 1.6968 mL | |
| 50 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3574 mL | |
| 60 mM | 0.0452 mL | 0.2262 mL | 0.4525 mL | 1.1312 mL | |
| 80 mM | 0.0339 mL | 0.1697 mL | 0.3394 mL | 0.8484 mL | |
| 100 mM | 0.0271 mL | 0.1357 mL | 0.2715 mL | 0.6787 mL |
IOX5 Related Classifications
HY-158341 Related Classifications
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