Jasplakinolide
Based on 10 publication(s) in Google Scholar
Jasplakinolide is a potent actin polymerization inducer and stabilizes pre-existing actin filaments. Jasplakinolide binds to F-actin competitively with phalloidin with a Kd of 15 nM. Jasplakinolide, a naturally occurring cyclic peptide from the marine sponge, has both fungicidal and anti-cancer activity.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity : 99.56%
- CAS No.: 102396-24-7
- 화학식: C36H45BrN4O6
- 분자량:709.67
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Jasplakinolide
More- Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
- EBioMedicine. 2026 Aug:130:106404.
- Chin Med J (Engl). 2024 Jan 20;137(2):209-221. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696. [Abstract]
- Cell Mol Life Sci. 2024 Sep 14;81(1):402. [Abstract]
- mBio. 2025 Oct 8;16(10):e0194525. [Abstract]
- Viruses. 2022 Jan 14;14(1):153. [Abstract]
- Food Chem Toxicol. 2025 Nov 21:208:115861. [Abstract]
- Theoretical and Applied Mechanics Letters. 2025 Aug 22.
- bioRxiv. 2023 Feb 5.
Customer Validation & Images
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Cell Imaging/Staining
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IF
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Cell Imaging/Staining
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2D/3D Cell Culture and Differentiation
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2D/3D Cell Culture and Differentiation
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | GI50 |
0.02 μM
Compound: 1
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Growth inhibition of human 786-0 cells by sulforhodamine B assay
Growth inhibition of human 786-0 cells by sulforhodamine B assay
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[PMID: 20121114] |
| A498 | GI50 |
0.03 μM
Compound: 1
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Anticancer activity against human A498 cells
Anticancer activity against human A498 cells
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[PMID: 21241058] |
| CA46 | IC50 |
0.03 μM
Compound: 5
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Antiproliferative activity against human CA46 cells after 24 hrs
Antiproliferative activity against human CA46 cells after 24 hrs
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[PMID: 12088429] |
| CA46 | IC50 |
10 nM
Compound: 1
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Growth inhibition of human CA46 cells assessed as induction of actin assembly after 24 hrs
Growth inhibition of human CA46 cells assessed as induction of actin assembly after 24 hrs
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[PMID: 20678932] |
| DU-145 | GI50 |
0.03 μM
Compound: 1
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Anticancer activity against human DU145 cells
Anticancer activity against human DU145 cells
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[PMID: 21241058] |
| HCT-116 | GI50 |
0.04 μM
Compound: 1
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Anticancer activity against human HCT116 cells
Anticancer activity against human HCT116 cells
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[PMID: 21241058] |
| HCT-116 | GI50 |
0.1 μM
Compound: 1
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Growth inhibition of human HCT116 cells by sulforhodamine B assay
Growth inhibition of human HCT116 cells by sulforhodamine B assay
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[PMID: 20121114] |
| HCT-15 | GI50 |
0.69 μM
Compound: 1
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Growth inhibition of human HCT15 cells by sulforhodamine B assay
Growth inhibition of human HCT15 cells by sulforhodamine B assay
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[PMID: 20121114] |
| HOP-62 | GI50 |
0.15 μM
Compound: 1
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Growth inhibition of human HOP62 cells by sulforhodamine B assay
Growth inhibition of human HOP62 cells by sulforhodamine B assay
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[PMID: 20121114] |
| IGROV-1 | GI50 |
0.02 μM
Compound: 1
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Anticancer activity against human IGROV1 cells
Anticancer activity against human IGROV1 cells
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[PMID: 21241058] |
| KB | IC50 |
0.013 μM
Compound: 1, jasplakinolide
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Growth inhibition of human KB cells after 72 hrs by CellTiter 96 assay
Growth inhibition of human KB cells after 72 hrs by CellTiter 96 assay
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[PMID: 22364566] |
| LNCaP | GI50 |
41 μM
Compound: 1
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Growth inhibition against human LNCAP cells assessed as disruption of actin cytoskeleton
Growth inhibition against human LNCAP cells assessed as disruption of actin cytoskeleton
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[PMID: 21241058] |
| LOX IMVI | GI50 |
0.01 μM
Compound: 1
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Anticancer activity against human LOXIMVI cells
Anticancer activity against human LOXIMVI cells
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[PMID: 21241058] |
| M14 | GI50 |
0.056 μM
Compound: 1
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Growth inhibition of human M14 cells by sulforhodamine B assay
Growth inhibition of human M14 cells by sulforhodamine B assay
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[PMID: 20121114] |
| MDA-MB-231 | GI50 |
0.01 μM
Compound: 1
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Anticancer activity against human MDA-MB-231 cells
Anticancer activity against human MDA-MB-231 cells
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[PMID: 21241058] |
| NCI-H522 | GI50 |
0.03 μM
Compound: 1
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Anticancer activity against human NCI-H522 cells
Anticancer activity against human NCI-H522 cells
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[PMID: 21241058] |
| OVCAR-3 | GI50 |
0.04 μM
Compound: 1
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Growth inhibition of human OVCAR-3 cells by sulforhodamine B assay
Growth inhibition of human OVCAR-3 cells by sulforhodamine B assay
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[PMID: 20121114] |
| PC-3 | GI50 |
65 μM
Compound: 1
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Growth inhibition against human PC3 cells assessed as disruption of actin cytoskeleton
Growth inhibition against human PC3 cells assessed as disruption of actin cytoskeleton
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[PMID: 21241058] |
| SF-539 | GI50 |
0.3 μM
Compound: 1
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Growth inhibition of human SF539 cells by sulforhodamine B assay
Growth inhibition of human SF539 cells by sulforhodamine B assay
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[PMID: 20121114] |
| U-251 | GI50 |
0.07 μM
Compound: 1
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Anticancer activity against human U251 cells
Anticancer activity against human U251 cells
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[PMID: 21241058] |
In Vitro
Jasplakinolide has an IC50 of 35 nM for the antiproliferative effect of jasplakinolide on PC3 prostate carcinoma cells[2].
Jasplakinolide (10 μM; for 2 hours) generates binucleated cells when applied during mitosis. Jasplakinolide induces the formation of F-actin-containing apical extensions in Toxoplasma gondii tachyzoites[2].
Jasplakinolide (1 mM; for 60 min) induces the formation of actin-containing apical extensions in isolated Toxoplasma gondii tachyzoites fixed[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 102396-24-7
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Appearance Solid
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분자량 709.67
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화학식 C36H45BrN4O6
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Color White to off-white
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SMILES
BrC1=C(C2=CC=CC=C2N1)C[C@@H](N(C([C@@H](NC([C@H](C/C(C)=C/[C@@H]3C)C)=O)C)=O)C)C(N[C@](C(C=C4)=CC=C4O)([H])CC(O[C@H](C3)C)=O)=O
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (10)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. [Abstract]2025 Sep 17:e00358. PMID: 40959902
Jasplakinolide purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
E16.5 fetal mouse ovaries were treated with or without 5 µM Jasplakinolide for 4 days. Jasplakinolide treatment altered the size of the ovaries and the morphology of oocytes.
Jasplakinolide purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
E16.5 fetal mouse ovaries were treated with or without 5 µM Jasplakinolide for 4 days. F-actin showed abnormal aggregation in both multinucleated cysts and oocytes.
Jasplakinolide purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 17:e00358. [Abstract]
E16.5 fetal mouse ovaries were treated with or without 5 µM Jasplakinolide for 4 days. The proportion of double-nucleated oocytes increased significantly.
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Chin Med J (Engl)
Cell softness reveals tumorigenic potential via ITGB8/AKT/glycolysis signaling in a mice model of orthotopic bladder cancer. [Abstract]2024 Jan 20;137(2):209-221. PMID: 37390491
Jasplakinolide purchased from MedChemExpress. Usage Cited in: Chin Med J (Engl). 2024 Jan 20;137(2):209-221. [Abstract]
Immunofluorescent staining of F-actin and TRIM59 in T24 cells cultured in flask, 3D matrix gels or 3D matrix gels containing Jas (20 mmol/L).
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Cell Mol Gastroenterol Hepatol
2021;11(3):683-696. PMID: 33075564
Jasplakinolide purchased from MedChemExpress. Usage Cited in: Cell Mol Gastroenterol Hepatol. 2021;11(3):683-696. [Abstract]
Low doses of Jasplakinolide (actin polymerization inducer) attenuated Fg-induced VP, while both high doses of Jasplakinolide and Cytochalasin D (an inhibitor of actin polymerization) dramatically increased Fg-induced VP.
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Cell Mol Life Sci
Tropomodulin1 exacerbates inflammatory response in macrophages by negatively regulating LPS-induced TLR4 endocytosis. [Abstract]2024 Sep 14;81(1):402. PMID: 39276234 -
mBio
PRRSV promotes bacterial infection by remodeling actin cytoskeleton and cell membrane proteins. [Abstract]2025 Oct 8;16(10):e0194525. PMID: 40937850 -
Viruses
Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin ( polh) and p10 as a Component of Transcription Initiation Complex (TIC). [Abstract]2022 Jan 14;14(1):153. PMID: 35062357 -
Food Chem Toxicol
Targeting the MLKL-F-actin-NLRP3 axis attenuates arsenic-induced myocardial necroinflammation. [Abstract]2025 Nov 21:208:115861. PMID: 41275903 -
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용액&용해도
In Vitro:
DMSO : ≥ 2 mg/mL (2.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Phalloidin F-actin cytoskeleton staining
Phalloidin F-actin staining detects polymerized filamentous actin in fixed and permeabilized specimens by using fluorescent phalloidin or phalloidin-derived phallotoxins that bind actin filaments and generate a fluorescence microscopy readout corresponding to F-actin organization, including stress fibers, cortical actin, filament bundles, and tissue-specific actin networks. Phalloidin stabilizes F-actin by reducing actin subunit dissociation from filament ends, and fluorescent phallotoxins were established as tools for visualizing actin-containing structures in eukaryotic cells.
순도&문서
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Data Sheet (284 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Bubb MR, et al. Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. J Biol Chem. 1994 May 27;269(21):14869-71. [Content Brief]
[2]. Holzinger A, et al. Jasplakinolide: an actin-specific reagent that promotes actin polymerization. Methods Mol Biol. 2009;586:71-87. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4091 mL | 7.0455 mL | 14.0911 mL | 35.2276 mL |
Jasplakinolide Related Classifications
HY-P0027 Related Classifications
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