LysoTracker Red
Based on 18 publication(s) in Google Scholar
LysoTracker Red is a Red fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 577/590 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and gather on spherical organelles. It is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 95.0%
- CAS No.: 231946-72-8
- 화학식: C20H24BF2N5O
- 분자량:399.25
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) LysoTracker Red
More- Nat Biotechnol. 2026 Jan 5. [Abstract]
- J Control Release. 2026 Apr 10:392:114710. [Abstract]
- J Immunother Cancer. 2025 Feb 6;13(2):e010150. [Abstract]
- Mater Today Bio. 2025 Nov 7:35:102527. [Abstract]
- ACS Appl Mater Interfaces. 2025 Aug 6;17(31):44922-44938. [Abstract]
- Aging Cell. 2024 Jun 19:e14256. [Abstract]
- Eur J Pharm Biopharm. 2024 Sep 18:114503. [Abstract]
- PLoS Pathog. 2025 Jan 2;21(1):e1012800. [Abstract]
- iScience. 2024 Jul 25;27(8):110588. [Abstract]
- Biochem J. 2025 Dec 17;482(24):1973-1992. [Abstract]
- J Med Virol. 2025 Sep;97(9):e70578. [Abstract]
- ACS Chem Biol. 2024 May 17;19(5):1161-1168. [Abstract]
- Res Sq. 2026 Jun 21.
- Res Sq. 2026 May 19.
- bioRxiv. 2026 Mar 29.
- Res Sq. 2025 Dec 2.
- Preprints. 2025 Jul 8.
- Heliyon. 2023 Nov 22;9(12):e22605. [Abstract]
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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IF
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Preparation of LysoTracker Red working solution
1. Working solution preparation:
1.1 Prepare LysoTracker Red stock solution with DMSO.
1.2 Dilute storage solution to the working solution using a medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM;
Note: Please adjust the concentration of the LysoTracker Red working solution according to the actual situation, and use it now.
2. Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of LysoTracker Red working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
590
577
Chemical Information
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CAS No. 231946-72-8
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Appearance Solid
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분자량 399.25
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화학식 C20H24BF2N5O
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Color Brown to dark brown
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SMILES
O=C(CCC1=CC=C2C=C3C=CC(C4=CC=CN4)=[N]3[B+3]([F-])([N-]21)[F-])NCCN(C)C
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Synonyms
LysoTracker Red DND-99
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
Engineering bispecific exosome activators of T cells to target immune checkpoint inhibitor-resistant metastatic melanoma. [Abstract]2026 Jan 5. PMID: 41491256
LysoTracker Red purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2026 Jan 5. [Abstract]
Representative immunostaining images of BEAT treated tumor cells. Lysosomes were stained with LysoTracker Red (30 min), BEAT were stained with DiD (gray), and nuclear were stained with Hoechst (blue).
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J Control Release
Anti-CLEC7A nanobody in situ engineering promotes amyloid-β oligomers clearance by CAR-microglia to alleviate Alzheimer's disease pathology in mice. [Abstract]2026 Apr 10:392:114710. PMID: 41702507 -
J Immunother Cancer
Reinforcing cancer immunotherapy with engineered porous hollow mycobacterium tuberculosis loaded with tumor neoantigens. [Abstract]2025 Feb 6;13(2):e010150. PMID: 39915006
LysoTracker Red purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Feb 6;13(2):e010150. [Abstract]
Neoantigens labeled with FITC were co-cultured with BMDCs for 5 hours. Lysosomes were stained with LysoTracker Red (1:500; 30 min; 4℃) dye. Confocal microscopy was used to observe co-localization of the two fluorescence with cell nuclei (DAPI fluorescence).
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Mater Today Bio
A bufalin and CRISPR/Cas9 ribonucleoprotein-loaded calcium lactate nanomedicine for pyroptosis/apoptosis and synergistic cancer immunotherapy. [Abstract]2025 Nov 7:35:102527. PMID: 41322154
LysoTracker Red purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 7:35:102527. [Abstract]
Endosomal escape of CLBRP in CT26 cells was observed via CLSM at 4 and 12 h posttreatment. The colocalization of EGFP-labeled CLBRP with LysoTracker Red (20 min) -stained endosomes was analyzed.
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ACS Appl Mater Interfaces
Tumor-Targeted PLGA Nanospheres Enhance Therapeutic Effect of Lenvatinib in Hepatocellular Carcinoma via Photothermal and Photodynamic Therapy. [Abstract]2025 Aug 6;17(31):44922-44938. PMID: 40689931
LysoTracker Red purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Aug 6;17(31):44922-44938. [Abstract]
CLSM images depicted the process of lysosomal escape of nanoparticles within the cells. Red represented LysoTracker Red (6-12 h), green represented the IR780-labeled nanoparticles, and yellow regions indicated the colocalization of nanoparticles with lysosomes.
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Aging Cell
Aging-induced short-chain acyl-CoA dehydrogenase promotes age-related hepatic steatosis by suppressing lipophagy. [Abstract]2024 Jun 19:e14256. PMID: 38898632
LysoTracker Red purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Jun 19:e14256. [Abstract]
Representative immunofluorescence images showing the colocalization of lysosomes labeled with LysoTracker Red and lipid droplets labeled with the green lipid dye BODIPY (scale bar: 50 μm).
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Eur J Pharm Biopharm
Enhanced photodynamic therapy of curcumin using biodegradable PLGA coated mesoporous silica nanoparticles. [Abstract]2024 Sep 18:114503. PMID: 39303950 -
PLoS Pathog
2025 Jan 2;21(1):e1012800. PMID: 39746094 -
iScience
Intergenerational epigenetic inheritance mediated by MYS-2/MOF in the pathogenesis of Alzheimer's disease. [Abstract]2024 Jul 25;27(8):110588. PMID: 39220410 -
Biochem J
Opposite effects of spermidine and GC7 in cell culture are dictated by distinct molecular targets. [Abstract]2025 Dec 17;482(24):1973-1992. PMID: 41408852 -
J Med Virol
Pexophagy Triggered by Enterovirus 71 Modulates the Production of Pro-Inflammatory Cytokines Through Cholesterol Accumulation in Lysosomes. [Abstract]2025 Sep;97(9):e70578. PMID: 40856297 -
ACS Chem Biol
A Novel Lysosome Targeting Chimera for Targeted Protein Degradation via Split-and-Mix Strategy. [Abstract]2024 May 17;19(5):1161-1168. PMID: 38662199 -
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Heliyon
2023 Nov 22;9(12):e22605. PMID: 38107270
용액&용해도
DMSO : 5 mg/mL (12.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Price OT, et al. Quantitative fluorescence of 5-FU-treated fetal rat limbs using confocal laser scanning microscopy and Lysotracker Red. Cytometry A. 2003;53(1):9-21. [Content Brief]
[2]. Pereira LC, et al. An autophagic process is activated in HepG2 cells to mediate BDE-100-induced toxicity. Toxicology. 2017;376:59-65. [Content Brief]
[3]. Nagahama M, et al. Cellular vacuolation induced by Clostridium perfringens epsilon-toxin. FEBS J. 2011;278(18):3395-3407. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5047 mL | 12.5235 mL | 25.0470 mL | 62.6174 mL |
| 5 mM | 0.5009 mL | 2.5047 mL | 5.0094 mL | 12.5235 mL | |
| 10 mM | 0.2505 mL | 1.2523 mL | 2.5047 mL | 6.2617 mL |
LysoTracker Red Related Classifications
HY-D1300 Related Classifications
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