MAP3K14-IN-1
MAP3K14-IN-1 (Compound 173) is a MAP3K14 (NIK kinase) inhibitor with an IC50 of 1.8 nM. MAP3K14-IN-1 inhibits the autophosphorylation of MAP3K14 kinase. MAP3K14-IN-1 reduces the level of phosphorylated IKKα in cancer cells. MAP3K14-IN-1 inhibits the proliferation of multiple myeloma cells. MAP3K14-IN-1 can be used in research related to multiple myeloma.
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- CAS No.: 2113617-02-8
- 화학식: C29H31N7O2
- 분자량:509.61
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
- References
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Help & FAQs
All MAP3K Isoforms
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Biological Activity
제품 설명
IC50 & Target
[1]|
NIK/MAP3K14 1.8 nM (IC50) |
IKKα 1.3 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| JJN-3 | IC50 |
29 nM
Compound: 173
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Antiproliferative activity against human JJN-3 cells after 96 hrs by CellTiter-Glo luminescence assay
Antiproliferative activity against human JJN-3 cells after 96 hrs by CellTiter-Glo luminescence assay
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[PMID: 28947934] |
| KMS-12-BM | IC50 |
>10 μM
Compound: 173
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Antiproliferative activity against human KMS-12-BM cells after 96 hrs by CellTiter-Glo luminescence assay
Antiproliferative activity against human KMS-12-BM cells after 96 hrs by CellTiter-Glo luminescence assay
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[PMID: 28947934] |
In Vitro
MAP3K14-IN-1 inhibits autophosphorylation of recombinant MAP3K14/NIK kinase in cell-free assays, with an IC50 of 1.8 nM[1].
MAP3K14-IN-1 inhibits the level of p-IKKα in L363 multiple myeloma cells, with an IC50 of 1.3 nM[1].
MAP3K14-IN-1 inhibits the proliferation of JJN-3 multiple myeloma cells with an IC50 of 29 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2113617-02-8
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분자량 509.61
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화학식 C29H31N7O2
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SMILES
C(O)[C@]1(C)C=2C(=C(C#N)C=C(C2)C3=NC(NC4=CC(C#N)=C(CCN5CCOCC5)C=C4C)=NC=C3)NC1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MAP3K14-IN-1 Related Classifications
HY-124865 Related Classifications
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