MM-589 TFA
Based on 1 Customer Validation
MM-589 TFA is a potent inhibitor of WD repeat domain 5 (WDR5) and mixed lineage leukemia (MLL) protein-protein interaction. MM-589 binds to WDR5 with an IC50 of 0.90 nM and inhibits the MLL H3K4 methyltransferase activity with an IC50 of 12.7 nM.
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- Purity: 98.67%
- CAS No.: 2253167-09-6
- 화학식: C30H45F3N8O7
- 분자량:686.72
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보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
IC50: 0.90 nM (WDR5), 12.7 nM (HMT)[1]
Ki: <1 nM (WDR5)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
8.56 μM
Compound: 18; MM-589
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Cytotoxicity against human HL60 cells lacking MLL translocation assessed as cell growth inhibition after 4 days by WST8 assay
Cytotoxicity against human HL60 cells lacking MLL translocation assessed as cell growth inhibition after 4 days by WST8 assay
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[PMID: 28603984] |
| MOLM-13 | IC50 |
0.1 μM
Compound: 18; MM-589
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Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 7 days by WST8 assay
Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 7 days by WST8 assay
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[PMID: 28603984] |
| MOLM-13 | IC50 |
0.21 μM
Compound: 18; MM-589
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Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay
Cytotoxicity against human MOLM13 cells harboring MLL-AF9 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay
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[PMID: 28603984] |
| MV4-11 | IC50 |
0.25 μM
Compound: 18; MM-589
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Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay
Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 4 days by WST8 assay
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[PMID: 28603984] |
| MV4-11 | IC50 |
0.29 μM
Compound: 18; MM-589
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Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 7 days by WST8 assay
Cytotoxicity against human MV4-11 cells harboring MLL-AF4 fusion protein assessed as cell growth inhibition after 7 days by WST8 assay
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[PMID: 28603984] |
MM-589 (0.01-10 μM, 4 days or 7 days) potently and selectively inhibits cell growth in human leukemia cell lines harboring MLL translocations[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MV4-11 and MOLM-13 cells
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Concentration:0.01, 0.1, 1, 10 μM
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Incubation Time:4 days or 7 days
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Result:Potently inhibited MV4-11 and MOLM-13 cell growth with IC50s of 0.25 and 0.21 μM, respectively. Had much weaker activity in the inhibition of cell growth of the HL-60 cell line with an IC50 of 8.6 μM.
Chemical Information
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CAS No. 2253167-09-6
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Appearance Solid
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분자량 686.72
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화학식 C30H45F3N8O7
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Color White to off-white
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SMILES
O=C(NCC[C@](NC(C(C)C)=O)(C)C1=O)[C@H](NC([C@@H](NC([C@H](CCCNC(NC)=N)N1)=O)CC)=O)C2=CC=CC=C2.FC(C(O)=O)(F)F
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
용액&용해도
DMSO : ≥ 100 mg/mL (145.62 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4562 mL | 7.2810 mL | 14.5620 mL | 36.4049 mL |
| 5 mM | 0.2912 mL | 1.4562 mL | 2.9124 mL | 7.2810 mL | |
| 10 mM | 0.1456 mL | 0.7281 mL | 1.4562 mL | 3.6405 mL | |
| 15 mM | 0.0971 mL | 0.4854 mL | 0.9708 mL | 2.4270 mL | |
| 20 mM | 0.0728 mL | 0.3640 mL | 0.7281 mL | 1.8202 mL | |
| 25 mM | 0.0582 mL | 0.2912 mL | 0.5825 mL | 1.4562 mL | |
| 30 mM | 0.0485 mL | 0.2427 mL | 0.4854 mL | 1.2135 mL | |
| 40 mM | 0.0364 mL | 0.1820 mL | 0.3640 mL | 0.9101 mL | |
| 50 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7281 mL | |
| 60 mM | 0.0243 mL | 0.1213 mL | 0.2427 mL | 0.6067 mL | |
| 80 mM | 0.0182 mL | 0.0910 mL | 0.1820 mL | 0.4551 mL | |
| 100 mM | 0.0146 mL | 0.0728 mL | 0.1456 mL | 0.3640 mL |
MM-589 TFA Related Classifications
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