OI-3

Compared with mouse-derived OI-3 (mOI-3), chimeric OI-3 (chOI-3) exhibits higher apparent affinity for CD146 on the surface of human pleural mesothelioma cells MSTO-211H and NCI-H226; the K_DAPP values of chOI-3 are 3.3 nM and 5.9 nM, respectively, while those of mOI-3 are 9.2 nM and 11.4 nM^[1].
mOI-3 and chOI-3 (0.25 μg; 1-24 h) are efficiently internalized into MSTO-211H human mesothelioma cells, reaching the internalization peak at 6-24 h; in contrast, chOI-3 shows extremely weak internalization into NCI-H226 human mesothelioma cells within 24 h^[1].
chOI-3 (0.25 μg/mL; 16 h) internalizes into the human pleural mesothelioma cell line MSTO-211H within 16 h, and its internalization rate in real-time live-cell imaging assays is significantly higher than that of the human IgG1 isotype control^[1].
²¹²Pb-TCMC-chOI-3 (5-10 kBq/mL; 2-24 h) is internalized into MSTO-211H human pleural mesothelioma cells. At 24 h, the number of internalized ²¹²Pb and ²¹²Bi atoms per cell is significantly higher than that at 2 h, reaching 13.7-fold and 8.5-fold of that of the isotype control, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.