(S)-Pulegone
(S)-Pulegone ((-)-Pulegone) is a cytochrome P450 (CYP450) substrate. (S)-Pulegone induces urothelial cytotoxicity, necrosis, regenerative proliferation and urinary bladder tumors in female rats. (S)-Pulegone induces rodent nephropathy, olfactory metaplasia, gastric hyperplasia/perforation at high dose. (S)-Pulegone can be used for the research of urinary bladder and hepatic neoplasms.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 3391-90-0
- 화학식: C10H16O
- 분자량:152.24
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
Biological Activity
제품 설명
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CAKI-1 | LC50 |
359 μM
Compound: 13
|
Lethal concentration required to kill 50% C33A cells of the human cervical cells with a mutation at Cys 273
Lethal concentration required to kill 50% C33A cells of the human cervical cells with a mutation at Cys 273
|
[PMID: 15482940] |
Chemical Information
-
CAS No. 3391-90-0
-
분자량 152.24
-
화학식 C10H16O
-
SMILES
C(C)(C)=C1C(=O)C[C@@H](C)CC1
-
Synonyms
(-)-Pulegone
-
Structure Classification
-
Initial Source
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(S)-Pulegone Related Classifications
HY-N1500A Related Classifications
Powered by Bioz