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Cat. No.: HY-161794

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SP3N

SP3N is a specific degrader of the prolyl isomerase FKBP12. As a prodrug, SP3N is metabolized by Diamine oxidase into the active aldehyde metabolite SP3CHO (HY-161795). SP3N induces the proximity of FKBP12 to the SCF^FBXO22 E3 ligase complex, thereby triggering the polyubiquitination and proteasomal degradation of FKBP12. SP3N can be used in cancer research.

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화학식: C₃₉H₅₇N₃O₁₀
분자량:727.88

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

SP3N Chemical Structure

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

^[1]

FKBP12

In Vitro

SP3N (1 nM-10 μM; 1-16 h) potently degrades FKBP12 in KBM7 iCas9 FKBP12-BFP-P2A-mCherry reporter cells in a time- and dose-dependent manner, and this degradation depends on the ubiquitin-proteasome system and Cullin-RING ligase activity^[1].
SP3N (1 μM; 16 h) degrades FKBP12 in HEK293T cells, with no detectable off-target degradation across the proteome after 16 h of 1 μM treatment^[1].
SP3N (0.001-20 μM; 16 h) dose-dependently reduces FKBP12 protein levels in HEK293T Nluc-3xFlag-FKBP12-NLS cells after 16 h of treatment^[1].
SP3N requires metabolic conversion by amine oxidases (such as DAO) to an active species to induce FKBP12 degradation in KBM7 iCas9 FKBP12-BFP-P2A-mCherry reporter cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK293T Nluc-3xFlag-FKBP12-NLS cells
Concentration:

0.001 μM, 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM
Incubation Time:

16 h
Result:

Reduced FKBP12 protein levels in a dose-dependent manner.
Started detectable degradation at 0.01 μM.
Achieved near-complete degradation at 20 μM.

Chemical Information

분자량 727.88
화학식 C₃₉H₅₇N₃O₁₀
SMILES

COC1=CC=C(CC[C@@H](OC([C@@H]2CCCCN2C(C(C(C)(C)CC)=O)=O)=O)C3=CC(NC(CCOCCOCCOCCN)=O)=CC=C3)C=C1OC
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kagiou C, et al. Alkylamine-tethered molecules recruit FBXO22 for targeted protein degradation. Nature communications. 2024 Jun 26;15(1):5409. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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