L18-MDP
Based on 1 Customer Validation
L18-MDP is a derivative of muramyl dipeptide, an antibacterial agent. L18-MDP has antibacterial activity and has potential applications in bacterial and fungal infections.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 60398-08-5
- Formula: C37H66N4O12
- Molecular Weight:758.94
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
L18-MDP (100 µg or 1000 µg; s.c., i.p., i.v. or p.o.; single dose) increases the survival rate of mice infected with Escherichia coli and improves the pathological tissue of mice infected with Listeria monocytogenes[1].
L18-MDP (100 µg or 200 µg; s.c. or i.p.; once daily for 3 days) increases the survival rate of mice infected with Candida albicans[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice infected with Escherichia coli, Klebsiella pneumoniae or Listeria monocytogenes[1].
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Dosage:100 µg or 1000 µg.
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Administration:Subcutaneous injection, intraperitoneal injection, intravenous injection or oral gavage; single dose.
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Result:Strengthened the phagocytosis of the microorganisms, weakened the virus activity, increased the survival rate and improved the pathological tissue.
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Animal Model:Mice infected with Candida albicans[1].
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Dosage:100 µg or 200 µg.
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Administration:Subcutaneous injection or intraperitoneal injection; once daily for 3 days.
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Result:Strengthened the phagocytosis of the microorganisms, weakened the virus activity, increased the survival rate and improved the pathological tissue.
Chemical Information
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CAS No. 60398-08-5
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Appearance Solid
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Molecular Weight 758.94
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Formula C37H66N4O12
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Color White to off-white
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Sequence
Ac-(6-O-stearoyl)-muramyl-Ala-{d-Glu}-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (131.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3176 mL | 6.5881 mL | 13.1763 mL | 32.9407 mL |
| 5 mM | 0.2635 mL | 1.3176 mL | 2.6353 mL | 6.5881 mL | |
| 10 mM | 0.1318 mL | 0.6588 mL | 1.3176 mL | 3.2941 mL | |
| 15 mM | 0.0878 mL | 0.4392 mL | 0.8784 mL | 2.1960 mL | |
| 20 mM | 0.0659 mL | 0.3294 mL | 0.6588 mL | 1.6470 mL | |
| 25 mM | 0.0527 mL | 0.2635 mL | 0.5271 mL | 1.3176 mL | |
| 30 mM | 0.0439 mL | 0.2196 mL | 0.4392 mL | 1.0980 mL | |
| 40 mM | 0.0329 mL | 0.1647 mL | 0.3294 mL | 0.8235 mL | |
| 50 mM | 0.0264 mL | 0.1318 mL | 0.2635 mL | 0.6588 mL | |
| 60 mM | 0.0220 mL | 0.1098 mL | 0.2196 mL | 0.5490 mL | |
| 80 mM | 0.0165 mL | 0.0824 mL | 0.1647 mL | 0.4118 mL | |
| 100 mM | 0.0132 mL | 0.0659 mL | 0.1318 mL | 0.3294 mL |
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