Lacto-N-neotetraose
Based on 1 Customer Validation
Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 13007-32-4
- Formula: C26H45NO21
- Molecular Weight:707.63
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Endogenous Metabolite[1], TNFR1[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) induces IL-8 secretion in T84 cells[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) reduces TNF-α induced IL-8-secretion with 38% in FHs 74 Int cells[2].
Lacto-N-neotetraose binds TNFR1 (Tumor necrosis factor receptor 1) with a Kd value of 900 ± 660 nM[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) attenuates TNF-α induced inflammation by TNFR1 ectodomain shedding in FHs 74 Int cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Lacto-N-neotetraose (100/200 μg, Intradermal injection, at 3, 7, 14, and 21 days post-surgery) increases the wound closure rate on day 7 post-wounding[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rabbit model of pneumonia[3]
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Dosage:100 μM
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Administration:Intratracheal administration for 24 h
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Result:Decreased by ~2 logs the bacterial load in the lung at 48 h after challenge.
Eliminated the extensive, edematous right middle lobe lesion evident at 48 h in control animals.
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Animal Model:Mice with symmetric full-thickness wounds
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Dosage:100, 200 μg
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Administration:Intradermal injection, at 3, 7, 14, and 21 days post-surgery.
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Result:Exhibited better healing score, follicle formation, and lower epidermal thickness index (H&E staining).
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 13007-32-4
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Appearance Solid
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Molecular Weight 707.63
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Formula C26H45NO21
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Color White to off-white
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SMILES
CC(N[C@H]([C@H]([C@@H]([C@H](O1)CO)O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)CO)O)[C@@H]1O[C@@H]3[C@H]([C@@H](O[C@@H]([C@@H]3O)CO)O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O)=O
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Synonyms
LNnT
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Structure Classification
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Initial Source
human
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 116.67 mg/mL (164.87 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiaomin Dong, et al. CRISPRi-Guided Multiplexed Fine-Tuning of Metabolic Flux for Enhanced Lacto- N-neotetraose Production in Bacillus subtilis. J Agric Food Chem. 2020 Feb 26;68(8):2477-2484. [Content Brief]
[2]. Lianghui Cheng, et al. The Human Milk Oligosaccharides 3-FL, Lacto-N-Neotetraose, and LDFT Attenuate Tumor Necrosis Factor-α Induced Inflammation in Fetal Intestinal Epithelial Cells In Vitro through Shedding or Interacting with Tumor Necrosis Factor Receptor 1. Mol Nutr Food Res. 2021 Apr;65(7):e2000425. [Content Brief]
[3]. I Idänpään-Heikkilä, et al. Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis. 1997 Sep;176(3):704-12. [Content Brief]
[4]. Behrouz Farhadihosseinabadi, et al. The in vivo effect of Lacto-N-neotetraose (LNnT) on the expression of type 2 immune response involved genes in the wound healing process. Sci Rep. 2020 Jan 22;10(1):997. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 1.4132 mL | 7.0658 mL | 14.1317 mL | 35.3292 mL |
| 5 mM | 0.2826 mL | 1.4132 mL | 2.8263 mL | 7.0658 mL | |
| 10 mM | 0.1413 mL | 0.7066 mL | 1.4132 mL | 3.5329 mL | |
| 15 mM | 0.0942 mL | 0.4711 mL | 0.9421 mL | 2.3553 mL | |
| 20 mM | 0.0707 mL | 0.3533 mL | 0.7066 mL | 1.7665 mL | |
| 25 mM | 0.0565 mL | 0.2826 mL | 0.5653 mL | 1.4132 mL | |
| 30 mM | 0.0471 mL | 0.2355 mL | 0.4711 mL | 1.1776 mL | |
| 40 mM | 0.0353 mL | 0.1766 mL | 0.3533 mL | 0.8832 mL | |
| 50 mM | 0.0283 mL | 0.1413 mL | 0.2826 mL | 0.7066 mL | |
| 60 mM | 0.0236 mL | 0.1178 mL | 0.2355 mL | 0.5888 mL | |
| 80 mM | 0.0177 mL | 0.0883 mL | 0.1766 mL | 0.4416 mL | |
| 100 mM | 0.0141 mL | 0.0707 mL | 0.1413 mL | 0.3533 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Lacto-N-neotetraose Related Classifications
HY-N9445 Related Classifications
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