2. Metabolic Enzyme/Protease Apoptosis
  3. Endogenous Metabolite TNF Receptor
  4. Lacto-N-neotetraose

Lacto-N-neotetraose  (Synonyms: LNnT)

Cat. No.: HY-N9445 Purity: 99.71%
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Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure.

For research use only. We do not sell to patients.

Lacto-N-neotetraose Chemical Structure

Lacto-N-neotetraose Chemical Structure

CAS No. : 13007-32-4

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5 mg USD 550 In-stock
10 mg USD 890 In-stock
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Based on 1 publication(s) in Google Scholar

Lacto-N-neotetraose Related Antibodies

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  • Purity & Documentation

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Description

Lacto-N-neotetraose (LNnT) is an endogenous metabolite. Lacto-N-neotetraose can inhibit TNF-α induced IL-8 secretion in immature epithelial cells. Lacto-N-neotetraose has anti-inflammatory avtivity, and can improve the wound closure[1][2][4].

IC50 & Target

Endogenous Metabolite[1], TNFR1[2].
In Vitro

Lacto-N-neotetraose (5 mg/mL, 24 h) induces IL-8 secretion in T84 cells[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) reduces TNF-α induced IL-8-secretion with 38% in FHs 74 Int cells[2].
Lacto-N-neotetraose binds TNFR1 (Tumor necrosis factor receptor 1) with a Kd value of 900 ± 660 nM[2].
Lacto-N-neotetraose (5 mg/mL, 24 h) attenuates TNF-α induced inflammation by TNFR1 ectodomain shedding in FHs 74 Int cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Lacto-N-neotetraose Related Antibodies
In Vivo

Lacto-N-neotetraose (100 μM, intratracheal administration for 24 h) reduces the abundance of Streptococcus pneumonia in the lungs of pneumonia rabbits[3].
Lacto-N-neotetraose (100/200 μg, Intradermal injection, at 3, 7, 14, and 21 days post-surgery) increases the wound closure rate on day 7 post-wounding[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rabbit model of pneumonia[3]
Dosage: 100 μM
Administration: Intratracheal administration for 24 h
Result: Decreased by ~2 logs the bacterial load in the lung at 48 h after challenge.
Eliminated the extensive, edematous right middle lobe lesion evident at 48 h in control animals.
Animal Model: Mice with symmetric full-thickness wounds
Dosage: 100, 200 μg
Administration: Intradermal injection, at 3, 7, 14, and 21 days post-surgery.
Result: Exhibited better healing score, follicle formation, and lower epidermal thickness index (H&E staining).
Molecular Weight

707.63

Formula

C26H45NO21
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(N[C@H]([C@H]([C@@H]([C@H](O1)CO)O[C@@H]2O[C@@H]([C@@H]([C@@H]([C@H]2O)O)O)CO)O)[C@@H]1O[C@@H]3[C@H]([C@@H](O[C@@H]([C@@H]3O)CO)O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O)=O
Structure Classification
Initial Source

human
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (176.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4132 mL 7.0658 mL 14.1317 mL
5 mM 0.2826 mL 1.4132 mL 2.8263 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.4132 mL 7.0658 mL 14.1317 mL 35.3292 mL
5 mM 0.2826 mL 1.4132 mL 2.8263 mL 7.0658 mL
10 mM 0.1413 mL 0.7066 mL 1.4132 mL 3.5329 mL
15 mM 0.0942 mL 0.4711 mL 0.9421 mL 2.3553 mL
20 mM 0.0707 mL 0.3533 mL 0.7066 mL 1.7665 mL
25 mM 0.0565 mL 0.2826 mL 0.5653 mL 1.4132 mL
30 mM 0.0471 mL 0.2355 mL 0.4711 mL 1.1776 mL
40 mM 0.0353 mL 0.1766 mL 0.3533 mL 0.8832 mL
50 mM 0.0283 mL 0.1413 mL 0.2826 mL 0.7066 mL
60 mM 0.0236 mL 0.1178 mL 0.2355 mL 0.5888 mL
80 mM 0.0177 mL 0.0883 mL 0.1766 mL 0.4416 mL
100 mM 0.0141 mL 0.0707 mL 0.1413 mL 0.3533 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Lacto-N-neotetraose Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lacto-N-neotetraose13007-32-4LNnTEndogenous MetaboliteTNF ReceptorTumor Necrosis Factor ReceptorTNFROligosaccharidesT84 cellFHs 74 IntinflammationpneumoniaHMOwound closureInhibitorinhibitorinhibit

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