LC-U7-44
LC-U7-44 is a potent USP7 inhibitor with an IC50 of 0.96 μM. LC-U7-44 binds to the hydrophobic catalytic pocket of USP7, forming hydrogen bonds with Gln297, Arg408, Asp295, Val296, and Gln351, and hydrophobic interactions with Leu406. LC-U7-44 exerts anti-proliferative effects on prostate cancer cells. LC-U7-44 can be used for the research of prostate cancer.
For research use only. We do not sell to patients.
- Formula: C28H32N6O3
- Molecular Weight:500.59
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
USP7 0.96 μM (IC50) |
In Vitro
LC-U7-44 (10 μM; 20-30 min) potently inhibits purified USP7 protein with an IC50 of 0.96 μM in a fluorescence polarization binding assay[1].
LC-U7-44 potently inhibits the enzymatic activity of purified USP7 protein with an IC50 of 0.46 μM in a Ub-Rho assay[1].
LC-U7-44 (0.01-100 μM; 6 days) potently inhibits the proliferation of LNCaP cells with an IC50 of 1.83 μM following 6 days of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:lymph node carcinoma of the prostate (LNCaP) cells
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Concentration:0.01-100 μM
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Incubation Time:6 days
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Result:Suppressed LNCaP cell proliferation with an IC50 of 1.83 μM.
Chemical Information
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Molecular Weight 500.59
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Formula C28H32N6O3
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SMILES
CN1N=C2C(N=CN(CC3(O)CCN(C(CCC4=CC=CC=C4)=O)CC3)C2=O)=C1C5=CC=C(CN)C=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
LC-U7-44 Related Classifications
HY-183282 Related Classifications
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