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Cat. No.: HY-187428

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LMed 99

LMed 99 is an antiviral agent. LMed 99 inhibits viral RNA synthesis and reduces viral protein expression in a time-dependent manner. LMed 99 potently inhibits Influenza A virus replication. LMed 99 can be used in the research of Influenza A virus infections (H1N1, H5N1).

For research use only. We do not sell to patients.

Formula: C₁₈H₁₁ClO₅
Molecular Weight:342.73

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

LMed 99 Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MDCK-II	CC₅₀	140.93 μM	Cytotoxicity against Canis familiaris distal renal tube epithelial MDCK II cells assessed via MTT assay with 72 h incubation. Cytotoxicity against Canis familiaris distal renal tube epithelial MDCK II cells assessed via MTT assay with 72 h incubation.	42485991
MDCK-II	EC50	1.21 μM	Inhibition of Influenza A virus H1N1 (strain WSN/33/H1N1) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus. Inhibition of Influenza A virus H1N1 (strain WSN/33/H1N1) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.	42485991
MDCK-II	EC50	4.91 μM	Inhibition of Influenza A virus H5N1 (strain A/Thailand/KAN-1/2004) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus. Inhibition of Influenza A virus H5N1 (strain A/Thailand/KAN-1/2004) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.	42485991

In Vitro

The CC₅₀ of LMed 99 (72 h) in MDCK II cells is 140.93 μM, indicating that it exhibits moderate cytotoxicity at high concentrations^[1].
LMed 99 (0.4-350 μM; 24 h) potently inhibits the replication of H1N1 in MDCK II cells, with an EC₅₀ of 1.21 μM and a selectivity index as high as 116.4^[1].
LMed 99 (9 μM) significantly reduces H1N1 vRNA accumulation at all tested post-infection time points in MDCK II cells, and inhibits viral mRNA synthesis during the early stages of infection (1 and 3 hpi)^[1].
LMed 99 (4.5-9 μM; added 4 h post-transfection and incubated for 20 h) enhances the activity of influenza A virus polymerase-related reporter genes in the MDCK II cell minireplicon system, with a 61.37% increase in activity at 4.5 μM and a 108.97% increase at 9 μM^[1].
LMed 99 (1.09-17.5 μM) inhibits the replication of highly pathogenic H5N1 in MDCK II cells, with an EC₅₀ of 4.91 μM and a selectivity index of 28.71, showing favorable performance^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

MDCK II cells
Concentration:

9 μM
Incubation Time:

added at 1 hpi, 3 hpi, or 6 hpi, with incubation until 9 hpi
Result:

Reduced M1 vRNA levels by 82% (n-fold = 0.18) at 1 and 3 hpi, and by 80% (n-fold = 0.20) at 6 hpi.
Reduced M1 mRNA levels by 78% (n-fold = 0.22) at 1 hpi and 70% (n-fold = 0.30) at 3 hpi, with no significant effect at 6 hpi.
Reduced PB1 mRNA levels by 57% (n-fold = 0.43) at 1 hpi and 22% (n-fold = 0.78) at 3 hpi, with no significant effect at 6 hpi.

Chemical Information

Molecular Weight 342.73
Formula C₁₈H₁₁ClO₅
SMILES

ClC1=CC(C=C2C(/C=C/C3=CC=C(C(O)=C3)O)=O)=C(C=C1)OC2=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Darido MLG, et al. Integrated in silico and in vitro approaches reveal novel coumarin hybrids targeting influenza A virus RNA-dependent RNA polymerase. European journal of medicinal chemistry. 2026 Jul 19;318:119172.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

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LMed 99

All DNA/RNA Synthesis Isoforms