2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. LR004

LR004 is an EGFR monoclonal antibody, with a Kd of 2.80 × 10-9 M against human EGFR. LR004 shows extremely weak inhibitory effect on the viability of EGFR-positive tumor cells in vitro, but inhibits the growth of EGFR-positive tumor xenografts as a single agent. LR004 is applicable to research related to advanced colorectal cancer, solid tumors, esophageal squamous cell carcinoma, epidermoid carcinoma, colon cancer and breast cancer.

For research use only. We do not sell to patients.

LR004

LR004 Chemical Structure

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Description

LR004 is an EGFR monoclonal antibody, with a Kd of 2.80 × 10-9 M against human EGFR. LR004 shows extremely weak inhibitory effect on the viability of EGFR-positive tumor cells in vitro, but inhibits the growth of EGFR-positive tumor xenografts as a single agent. LR004 is applicable to research related to advanced colorectal cancer, solid tumors, esophageal squamous cell carcinoma, epidermoid carcinoma, colon cancer and breast cancer[1][2].

Species Reactivity

Human
In Vitro

LR004 (5.0 μg/mL) binds recombinant human EGFR antigen with moderate affinity, with a Kd of 2.80 × 10-9 M[1].
LR004 (0.023-50 μg/mL; 24 h initial incubation, followed by 96 h incubation with serum-containing medium) inhibits the proliferation of MDA-MB-468 human breast cancer cells with an EC50 of 2.278 μg/mL[1].
LR004 (0.001-1 μg·mL-1; 48 h) has weak in vitro cytotoxicity against human EGFR-positive tumor cells (KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, AsPC-1) and EGFR-negative Karpas 299 cells, with IC50 values >6 nM or >60 nM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LR004 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-468 human breast cancer cells
Concentration: 0.023-50 μg/mL
Incubation Time: 24 h (initial incubation); 96 h (incubation with serum-containing medium)
Result: Inhibited MDA-MB-468 cell proliferation with an EC50 of 2.278 μg/mL.

Cell Cytotoxicity Assay[2]

Cell Line: KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, AsPC-1, Karpas 299
Concentration: 0.001-1 μg·mL-1
Incubation Time: 48 h
Result: Had a weak inhibitory effect on cell viability, with IC50 values >6 nM for KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, and AsPC-1 cells, and IC50 >60 nM for Karpas 299 cells.
In Vivo

LR004 (15 mg/kg; i.v.; every 4 days; 4 total doses) induces minimal tumor growth inhibition, with a 13.8% tumor inhibition rate, in KYSE520 esophageal squamous cell carcinoma xenografts[2].
LR004 (15 mg/kg; i.v.; every 4 days; 6 total doses) exerts limited tumor growth suppression in A431 epidermoid carcinoma xenografts[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude (female, 6-8 weeks old, 18-20 g, KYSE520 tumor cell xenograft)[2]
Dosage: 15 mg/kg
Administration: i.v.; every 4 days; 4 total doses
Result: Achieved an average tumor volume of 1791 mm3 on day 44.
Induced a 13.8% tumor inhibition rate.
Showed no tumor regression.
Caused no body weight loss or mortality.
Detected no histopathological abnormalities in major organs.
Animal Model: BALB/c nude (female, 6-8 weeks old, 18-20 g, A431 tumor cell xenograft)[2]
Dosage: 15 mg/kg
Administration: i.v.; every 4 days; 6 total doses
Result: Achieved an average tumor volume of 399 mm3 on day 60.
Exhibited limited therapeutic activity compared to LR004-VC-MMAE groups.
Detected no histopathological abnormalities in major organs.
Gene ID

1956  [NCBI]

Accession

P00533-1
Target

EGFR
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
SMILES

[LR004]
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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LR004 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LR004LR 004LR-004EGFREpidermal growth factor receptorErbB-1HER1rhesus monkeysMDA-MB-468 human breast cancer cellsA431 xenograft tumorsnude miceKYSE520 cellscolorectal carcinomalysosomesxenograft micehuman EGFRInhibitorinhibitorinhibit

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