2. Induced Disease Models Products Metabolic Enzyme/Protease Anti-infection Immunology/Inflammation NF-κB
  3. Immunology and Inflammatory Disease Models Endogenous Metabolite Glycosidase Bacterial Interleukin Related Reactive Oxygen Species (ROS)
  4. Arthritis Models
  5. Mannan

Mannan is an orally active polysaccharide compound that binds to the mannose receptor (MR). Mannan promotes bacterial uptake and endosomal degradation by binding to MR, thereby enhancing the production of IL-12 in immune cells. Mannan enhances ROS production. Mannan modulates immunity, inhibits Aflatoxin B1 (HY-N6615)-induced toxicity, and reduces lipid.

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Mannan

Mannan 화학구조

CAS No. : 9036-88-8

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고객리뷰

Based on 1 publication(s) in Google Scholar

Mannan Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Mannan

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Human Endogenous Metabolite Microbial Metabolite

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

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제품 설명

Mannan is an orally active polysaccharide compound that binds to the mannose receptor (MR). Mannan promotes bacterial uptake and endosomal degradation by binding to MR, thereby enhancing the production of IL-12 in immune cells. Mannan enhances ROS production. Mannan modulates immunity, inhibits Aflatoxin B1 (HY-N6615)-induced toxicity, and reduces lipid[1][2][3][4].

In Vitro

Mannan (10-100 μg/mL, 30 min) enhances the IL-12 production by increasing bacterial uptake and endosomal degradation in L. acidophilus and S. aureus stimulated dendritic cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mannan Related Antibodies

Western Blot Analysis[1]

Cell Line: Murine bone marrow-derived dendritic cells (BMDCs)
Concentration: 10 µg/mL, 100 µg/mL
Incubation Time: 30 min for pretreatment before bacteria stimulation; 60 min before adding bacteria for endocytosis assay; 1 h for MR surface expression assay
Result: Led to a decrease in the surface expression of the mannose receptor (MR) detected.

Real Time qPCR[1]

Cell Line: Murine bone marrow-derived dendritic cells (BMDCs)
Concentration: 10 µg/mL, 100 µg/mL
Incubation Time: 30 min for pretreatment before bacteria stimulation; 60 min before adding bacteria for endocytosis assay; 1 h for MR surface expression assay
Result: Increased the expression of IFN-β in cells stimulated with L. acidophilus.
In Vivo

Mannan (50-500 ppm; p.o., dietary; daily; 4 weeks) reduces Aflatoxin B1 (HY-N6615)-induced toxicity in mice, as evidenced by increased body weight, decreased rate of normochromatic erythrocytes with micronuclei, and decreased rate of sister chromatid exchanges[2].
Mannan (50-100 mg/kg; i.p.; 2-5 times) reduces serum levels of low-density lipoprotein (LDL), cholesterol, and triglycerides in mice with acute dyslipidemia induced by poloxamer 407 (HY-D1005)[4].

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

1. Induction of PsA[5][6][7]
Background
Mannan induces PsA by activating macrophages to produce TNF-α, which stimulates γδ T cells to secrete IL-17A, leading to neutrophil-driven skin and joint inflammation[5][6][7].
Specific Modeling Methods
Mice: B10Q and B10Q.Ncf1m1j/m1j mice
Administration: Mannan (10 or 20 mg in 200 μL PBS) • i.p. • either only on day 0 or for multiple exposures additionally on days 7 and 14
Mice: Pla1a-/- mice • 12-13 weeks
Administration: Mannan (20 mg in 200 μL PBS) • i.p. • dose on days 0, 4, and 8
Mice: EDIL3-knockout mice
Administration: Mannan (20 mg in 200 μL PBS) • i.p. • dose on days 1, 2, and 3
Note
Mice were weighed and monitored for clinical signs of PsA on day 0 and every two days after day 4. Arthritic paw scores were as follows: 0, no swelling and redness; 1, mild swelling and/or redness, flexibility present; 2, moderate swelling and/or redness, flexibility present; 3, moderate swelling and/or redness, little flexibility; 4; severe swelling and redness, no flexibility. Paw scaling score was as follows: 0, no; 1, mild; 2, moderate; 3, severe scaling.
Modeling Indicators
Molecular changes: Elevated levels of ROS, IL-17A, TNF-α, IL-6, IL-23, RANKL, and Trap
Cellular changes: Increased infiltration/activation of MoDCs, γδ T cells, and Th17 cells
Skin phenotype: Erythema, plaques, and scaling on paws and ears, Onycholysis and alopecia in affected areas
Histology analysis: Hyperkeratosis, acanthosis, and subcorneal microabscesses (neutrophil accumulation), Synovial hyperplasia, inflammatory infiltration, mild cartilage erosion, entheseal new bone formation, and osteoclasts (TRAP⁺)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male CBA/Lac mice (2.5-3-month-old, weighing 22-25 g), acute lipemia induced by poloxamer 407 model[4]
Dosage: 50 mg/kg, 100 mg/kg
Administration: Intraperitoneal injection, 5 times at 50 mg/kg every other day or 2 times at 100 mg/kg with 1-day interval
Result: Reduced the levels of triglyceride, atherogenic LDL, and total cholesterol in the serum at 50 mg/kg and 100 mg/kg.
Decreased the triglyceride concentration in the liver.
Increased the lability of lysosomal membranes in the liver and elevated the serum activity of chitotriosidase, a marker of macrophage activation.
Clinical Trial
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[Mannan]
Structure Classification
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

H2O : 50 mg/mL (Need ultrasonic)

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순도&문서

Purity: 98.4%

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Mannan Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mannan9036-88-8Endogenous MetaboliteGlycosidaseBacterialInterleukin RelatedReactive Oxygen Species (ROS)ILbiochemical assayreagentInhibitorinhibitorinhibit

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상품명:
Mannan
Cat. No.:
HY-W145667
수량:
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