MARK4 inhibitor 4
Based on 1 Customer Validation
MARK4 inhibitor 4 is a MARK4 inhibitor with an IC50 of 1.49 μM. MARK4 inhibitor 4 can be used in cancer and tau lesion-related research.
For research use only. We do not sell to patients.
- Purity: 99.23%
- Formula: C33H27ClN4O3
- Molecular Weight:563.05
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
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High-Throughput Bioactive Compound Library
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Biological Activity
IC50: 1.49 μM (MARK4)
MARK4 inhibitor 4 (Compound 23c) (1-20 μM; 24 h) has obvious inhibitory effect on the growth of Hela and U87MG cells, with EC50s value of 2.16 and 3.51 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hela, U87MG, U251, MDA-MB-435, HGFs cells
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Concentration:1-20 μM
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Incubation Time:24 h
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Result:Showed obvious cytotoxicity in Hela and U87MG cell lines.
Chemical Information
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Appearance Solid
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Molecular Weight 563.05
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Formula C33H27ClN4O3
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Color Light yellow to yellow
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SMILES
O=C(NC1=CC=C(Cl)C=C1)[C@@H](NC(CN(C2=C3C=CC=C2)C4=CC=C(C)C=C4C3=O)=O)CC5=CNC6=C5C=CC=C6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (177.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7760 mL | 8.8802 mL | 17.7604 mL | 44.4010 mL |
| 5 mM | 0.3552 mL | 1.7760 mL | 3.5521 mL | 8.8802 mL | |
| 10 mM | 0.1776 mL | 0.8880 mL | 1.7760 mL | 4.4401 mL | |
| 15 mM | 0.1184 mL | 0.5920 mL | 1.1840 mL | 2.9601 mL | |
| 20 mM | 0.0888 mL | 0.4440 mL | 0.8880 mL | 2.2201 mL | |
| 25 mM | 0.0710 mL | 0.3552 mL | 0.7104 mL | 1.7760 mL | |
| 30 mM | 0.0592 mL | 0.2960 mL | 0.5920 mL | 1.4800 mL | |
| 40 mM | 0.0444 mL | 0.2220 mL | 0.4440 mL | 1.1100 mL | |
| 50 mM | 0.0355 mL | 0.1776 mL | 0.3552 mL | 0.8880 mL | |
| 60 mM | 0.0296 mL | 0.1480 mL | 0.2960 mL | 0.7400 mL | |
| 80 mM | 0.0222 mL | 0.1110 mL | 0.2220 mL | 0.5550 mL | |
| 100 mM | 0.0178 mL | 0.0888 mL | 0.1776 mL | 0.4440 mL |
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