Anti-EphA2 Antibody (1C1)  (Synonyms: MEDI-547 antibody)

Anti-EphA2 Antibody (1C1) (MEDI-547 antibody) is an anti-EphA2 monoclonal antibody. Anti-EphA2 Antibody (1C1) can induce degradation of EphA2 protein. Anti-EphA2 Antibody (1C1) conjugated with McMMAF (HY-15578) can form an antibody-conjugated drugs, which has potent antitumor activity. Anti-EphA2 Antibody (1C1) can be used for the research of cancer, such as prostate cancer and glioma^[1][2].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

EphA2

Anti-EphA2 Antibody (1C1) shows human, mouse, rat, and cynomolgus monkey EphA2 binding affinities of 0.43, 0.92, 0.76 and 0.55 nM^[1].
Anti-EphA2 Antibody (1C1) (10 μg/mL; 6 h) induces degradation of EphA2 protein in PC3 human prostate cancer cells and F98 rat glioma cells^[1].
Anti-EphA2 Antibody (1C1) (72 h) has no effect on the viability of PC3 human prostate cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-EphA2 Antibody (1C1) (conjugated with mcMMAF) (0.1-3 mg/kg; i.v.; once weekly; 5 weeks) induces tumor stasis at 1 mg/kg, while 3 mg/kg induces tumor regressions in PC3 prostate tumor-bearing nude mice without observable adverse effects^[1].
Anti-EphA2 Antibody (1C1) (conjugated with mcMMAF) (2.5-10 mg/kg; i.v.; once weekly) significantly inhibits growth of EphA2-expressing F98 glioma tumors in Fischer 344 rats without observable adverse effects^[1].
Anti-EphA2 Antibody (1C1) (conjugated with mcMMAF) (5 mg/kg; i.v.; once weekly) does not inhibit growth of non-EphA2-expressing RG2 glioma tumors in Fischer 344 rats^[1].
Anti-EphA2 Antibody (1C1) (conjugated with mcMMAF) (1-5 mg/kg; i.v.; single dose) induces 60% EphA2 degradation in PC3 tumors by 1 hour at 1 mg/kg, while a 5 mg/kg single dose induces >90% EphA2 degradation maintained for 6 days in PC3 prostate tumor-bearing nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1969  [NCBI]

P29317

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

146.68 kDa

Liquid

Colorless to light yellow

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow Cytometry analysis of HepG2 cells with EphA2 (HY-P990627, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa(HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Immobilized EphA2 Protein, Human can bind MEDI-547. The EC₅₀ for this effect is 12.25 ng/mL.

• [1]. Jackson D, et al. A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo. Cancer Res. 2008 Nov 15;68(22):9367-74.  [Content Brief]

  [2]. Peng L, et al. Structural and functional characterization of an agonistic anti-human EphA2 monoclonal antibody. J Mol Biol. 2011;413(2):390-405.  [Content Brief]