Methyl L-pyroglutamate
Based on 1 Customer Validation
Methyl L-pyroglutamate ((S)-Methyl 5-oxopyrrolidine-2-carboxylate) is a phenolic compound. Methyl L-pyroglutamate can be isolated from Portulaca oleracea L. Methyl L-pyroglutamate slightly inhibits NO production. Methyl L-pyroglutamate can be used in the research of inflammatory diseases.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.62%
- No. CAS: 4931-66-2
- Fòrmula: C6H9NO3
- Peso molecular:143.14
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Almacenamiento:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-W012738
DL-Pyroglutamic acid -
HY-W002304
(R)-5-Oxopyrrolidine-2-carboxylic acid -
HY-W003321
(S)-tert-Butyl 5-oxopyrrolidine-2-carboxylate -
HY-76082
L-Pyroglutamic acid -
HY-76082R
L-Pyroglutamic acid (Standard) -
HY-W009673
Boc-Pyr-OH -
HY-41631
Boc-Pyr-Oet -
HY-W002304R
(R)-5-Oxopyrrolidine-2-carboxylic acid (Standard) -
HY-40098
(S)-1-Boc-3-aminopyrrolidine -
HY-32290
Methyl DL-pyroglutamate -
HY-40102
(S)-1-Boc-2-(aminomethyl)pyrrolidine -
HY-40269
L-Pyroglutamic acid ethyl ester
Actividad biológica
Descripciòn
In Vitro
Methyl L-pyroglutamate (0.5-100 μM) inhibits nitric oxide production in LPS-induced RAW 264.7 mouse macrophages, with an inhibition rate of less than 50%[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 4931-66-2
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Appearance Liquid (Density: 1.226 g/cm3)
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Peso molecular 143.14
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Fòrmula C6H9NO3
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Color Colorless to light yellow
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SMILES
O=C(OC)[C@H](CC1)NC1=O
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Synonyms
(S)-Methyl 5-oxopyrrolidine-2-carboxylate; L-Pyroglutamic acid methyl ester
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (698.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.47 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.9862 mL | 34.9308 mL | 69.8617 mL | 174.6542 mL |
| 5 mM | 1.3972 mL | 6.9862 mL | 13.9723 mL | 34.9308 mL | |
| 10 mM | 0.6986 mL | 3.4931 mL | 6.9862 mL | 17.4654 mL | |
| 15 mM | 0.4657 mL | 2.3287 mL | 4.6574 mL | 11.6436 mL | |
| 20 mM | 0.3493 mL | 1.7465 mL | 3.4931 mL | 8.7327 mL | |
| 25 mM | 0.2794 mL | 1.3972 mL | 2.7945 mL | 6.9862 mL | |
| 30 mM | 0.2329 mL | 1.1644 mL | 2.3287 mL | 5.8218 mL | |
| 40 mM | 0.1747 mL | 0.8733 mL | 1.7465 mL | 4.3664 mL | |
| 50 mM | 0.1397 mL | 0.6986 mL | 1.3972 mL | 3.4931 mL | |
| 60 mM | 0.1164 mL | 0.5822 mL | 1.1644 mL | 2.9109 mL | |
| 80 mM | 0.0873 mL | 0.4366 mL | 0.8733 mL | 2.1832 mL | |
| 100 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.7465 mL |
Methyl L-pyroglutamate Related Classifications
HY-32291 Related Classifications
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