メタンスルホン酸メチル
Based on 3 publication(s) in Google Scholar
Methyl methanesulfonate is an alkylating agent which transfers methyl groups, and induces DNA damage. Methyl methanesulfonate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.96%
- CAS 番号: 66-27-3
- 分子式: C2H6O3S
- 分子量:110.13
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保管条件:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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MedChemExpress（MCE）の使用を引用している文献 Methyl methanesulfonate
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Cell Proliferation/Viability Assay
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | EC50 |
2750 μM
Compound: MMS
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Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay
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[PMID: 21839641] |
体外実験
Methyl methanesulfonate (50-800 μM; 24 h) upregulates the generation of phosphorylated p53, RIPK and ROS, induces necroptosis in cell A549[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549
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Concentration:50-800 μM
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Incubation Time:24 h
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Result:Increased p53 phosphorylation and RIPK.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NMRI mouse model[2]
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Dosage:120 mg/kg
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Administration:ip, single dose
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Result:Increased DNA with comet tails in mouse liver, lung, kidney, and spleen.
化学情報
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CAS 番号 66-27-3
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性状 Liquid (Density: 1.3 g/cm3)
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分子量 110.13
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分子式 C2H6O3S
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Color Colorless to light yellow
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SMILES
COS(C)(=O)=O
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別名
メチルスルホン酸; Methyl methanesulfonate
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Sci Signal
PARP1-mediated PARylation of TEAD4 stabilizes the YAP1-TEAD4 complex and promotes growth and immune evasion in breast cancer cells. [Abstract]2025 Oct 21;18(909):eadx2532. PMID: 41118450 -
J Cell Mol Med
Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs. [Abstract]2019 Oct;23(10):6797-6804. PMID: 31338966
Methyl methanesulfonate purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2019 Oct;23(10):6797-6804. [Abstract]
Berberine sensitizes breast cancer cells (MDA-MB-231) to Methyl methanesulfonate (0-800 μmol/L).
溶剤 & 溶解度
体外:
H2O : ≥ 100 mg/mL (908.02 mM)
DMSO : 100 mg/mL (908.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
純度とドキュメンテーション
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データシート (282 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Jiang Y, et al., Methyl methanesulfonate induces necroptosis in human lung adenoma A549 cells through the PIG-3-reactive oxygen species pathway. Tumour Biol. 2016 Mar;37(3):3785-95. [Content Brief]
[2]. Hosseinzadeh H, et al., Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol. 2008 Dec;27(12):657-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 9.0802 mL | 45.4009 mL | 90.8018 mL | 227.0045 mL |
| 5 mM | 1.8160 mL | 9.0802 mL | 18.1604 mL | 45.4009 mL | |
| 10 mM | 0.9080 mL | 4.5401 mL | 9.0802 mL | 22.7004 mL | |
| 15 mM | 0.6053 mL | 3.0267 mL | 6.0535 mL | 15.1336 mL | |
| 20 mM | 0.4540 mL | 2.2700 mL | 4.5401 mL | 11.3502 mL | |
| 25 mM | 0.3632 mL | 1.8160 mL | 3.6321 mL | 9.0802 mL | |
| 30 mM | 0.3027 mL | 1.5134 mL | 3.0267 mL | 7.5668 mL | |
| 40 mM | 0.2270 mL | 1.1350 mL | 2.2700 mL | 5.6751 mL | |
| 50 mM | 0.1816 mL | 0.9080 mL | 1.8160 mL | 4.5401 mL | |
| 60 mM | 0.1513 mL | 0.7567 mL | 1.5134 mL | 3.7834 mL | |
| 80 mM | 0.1135 mL | 0.5675 mL | 1.1350 mL | 2.8376 mL | |
| 100 mM | 0.0908 mL | 0.4540 mL | 0.9080 mL | 2.2700 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Methyl methanesulfonate 関連分類
HY-W004702 関連分類
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