Methyl methanesulfonate
Based on 3 publication(s) in Google Scholar
Methyl methanesulfonate is an alkylating agent which transfers methyl groups, and induces DNA damage. Methyl methanesulfonate is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 66-27-3
- Formula: C2H6O3S
- Molecular Weight:110.13
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Methyl methanesulfonate
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Cell Proliferation/Viability Assay
Biological Activity
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Cell Line
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Type | Value | Description | References |
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| MCF7 | EC50 |
2750 μM
Compound: MMS
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Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 16 hrs by MTT assay
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[PMID: 21839641] |
Methyl methanesulfonate (50-800 μM; 24 h) upregulates the generation of phosphorylated p53, RIPK and ROS, induces necroptosis in cell A549[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549
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Concentration:50-800 μM
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Incubation Time:24 h
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Result:Increased p53 phosphorylation and RIPK.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NMRI mouse model[2]
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Dosage:120 mg/kg
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Administration:ip, single dose
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Result:Increased DNA with comet tails in mouse liver, lung, kidney, and spleen.
Chemical Information
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CAS No. 66-27-3
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Appearance Liquid (Density: 1.3 g/cm3)
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Molecular Weight 110.13
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Formula C2H6O3S
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Color Colorless to light yellow
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SMILES
COS(C)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Sci Signal
PARP1-mediated PARylation of TEAD4 stabilizes the YAP1-TEAD4 complex and promotes growth and immune evasion in breast cancer cells. [Abstract]2025 Oct 21;18(909):eadx2532. PMID: 41118450 -
J Cell Mol Med
Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs. [Abstract]2019 Oct;23(10):6797-6804. PMID: 31338966
Methyl methanesulfonate purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2019 Oct;23(10):6797-6804. [Abstract]
Berberine sensitizes breast cancer cells (MDA-MB-231) to Methyl methanesulfonate (0-800 μmol/L).
Solvent & Solubility
H2O : ≥ 100 mg/mL (908.02 mM)
DMSO : 100 mg/mL (908.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (22.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (791 KB)
- English - EN (791 KB)
- Français - FR (791 KB)
- Deutsch - DE (791 KB)
- Norwegian - NO (791 KB)
- Español - ES (791 KB)
- Swedish - SV (791 KB)
- Italian - IT (791 KB)
- Portuguese - PT (791 KB)
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Handling Instructions (2659 KB)
References
[1]. Jiang Y, et al., Methyl methanesulfonate induces necroptosis in human lung adenoma A549 cells through the PIG-3-reactive oxygen species pathway. Tumour Biol. 2016 Mar;37(3):3785-95. [Content Brief]
[2]. Hosseinzadeh H, et al., Protective effect of Crocus sativus stigma extract and crocin (trans-crocin 4) on methyl methanesulfonate-induced DNA damage in mice organs. DNA Cell Biol. 2008 Dec;27(12):657-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 9.0802 mL | 45.4009 mL | 90.8018 mL | 227.0045 mL |
| 5 mM | 1.8160 mL | 9.0802 mL | 18.1604 mL | 45.4009 mL | |
| 10 mM | 0.9080 mL | 4.5401 mL | 9.0802 mL | 22.7004 mL | |
| 15 mM | 0.6053 mL | 3.0267 mL | 6.0535 mL | 15.1336 mL | |
| 20 mM | 0.4540 mL | 2.2700 mL | 4.5401 mL | 11.3502 mL | |
| 25 mM | 0.3632 mL | 1.8160 mL | 3.6321 mL | 9.0802 mL | |
| 30 mM | 0.3027 mL | 1.5134 mL | 3.0267 mL | 7.5668 mL | |
| 40 mM | 0.2270 mL | 1.1350 mL | 2.2700 mL | 5.6751 mL | |
| 50 mM | 0.1816 mL | 0.9080 mL | 1.8160 mL | 4.5401 mL | |
| 60 mM | 0.1513 mL | 0.7567 mL | 1.5134 mL | 3.7834 mL | |
| 80 mM | 0.1135 mL | 0.5675 mL | 1.1350 mL | 2.8376 mL | |
| 100 mM | 0.0908 mL | 0.4540 mL | 0.9080 mL | 2.2700 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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