MFI8
Based on 12 publication(s) in Google Scholar
MFI8 is a small molecule inhibitor of mitochondrial.
For research use only. We do not sell to patients.
- Purity: 99.67%
- CAS No.: 694488-83-0
- Formula: C16H18ClNO
- Molecular Weight:275.77
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) MFI8
More- Signal Transduct Target Ther. 2025 Dec 17;10(1):413. [Abstract]
- Nat Commun. 2023 Jul 28;14(1):4557. [Abstract]
- J Adv Res. 2025 Nov 26:S2090-1232(25)00955-5. [Abstract]
- Biochem Pharmacol. 2024 Nov 5;230(Pt 3):116611. [Abstract]
- Radiother Oncol. 2024 Jan:190:110028. [Abstract]
- Exp Neurol. 2026 Apr:398:115636. [Abstract]
- J Cell Mol Med. 2025 Sep;29(18):e70861. [Abstract]
- Neuroscience. 2025 Sep 26:587:108-122. [Abstract]
- Biochem Biophys Res Commun. 2025 Sep 30:782:152609. [Abstract]
- Res Sq. 2026 May 5:rs.3.rs-9589283. [Abstract]
- Res Sq. 2026 Apr 24:rs.3.rs-9504540. [Abstract]
- bioRxiv. 2026 Feb 11:2026.02.10.704860. [Abstract]
Customer Validation & Images
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WB
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
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WB
Biological Activity
Mitofusin[1]
MFI8 (20 μM; 6?h) reduces significantly mitochondrial aspect ratio with an EC50 of 4.8 μM, and inhibits mitochondrial function via mitofusins and subsequently promoting mitochondrial fission[1].
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MFI8 possesses functional groups that meet the criteria of the pharmacophore model to interact with mitofusins and promotes mitochondrial fission[1].
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MFI8 binds to the HR2 domain of MFN2, modulates MFN conformation and complexes, and alters mitochondrial functionality[1].
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MFI8 (0-20 μM; 6 h) concentration-responsively increases caspase-3/7 activity, induces cytochrome c release and decreases membrane potential in a mitofusin-dependent manner. MFI8 also induces DNA damage and induces cell death in combination with BV6 SMAC (second mitochondria-derived activator of caspases) mimetic[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 694488-83-0
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Appearance Solid
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Molecular Weight 275.77
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Formula C16H18ClNO
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Color White to off-white
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SMILES
ClC1=CC=C(C(C(C)NC2=CC=CC(C)=C2C)=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (12)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Disruption of heme homeostasis by nuclear receptor Nur77 induces pyroptosis through granzyme B-dependent GSDMC cleavage. [Abstract]2025 Dec 17;10(1):413. PMID: 41407678 -
Nat Commun
Cancer cell-mitochondria hybrid membrane coated Gboxin loaded nanomedicines for glioblastoma treatment. [Abstract]2023 Jul 28;14(1):4557. PMID: 37507371
MFI8 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jul 28;14(1):4557. [Abstract]
The content of Gboxin in mitochondria isolated from U87MG cells, with or without MFI8 (5 μM; 6 h) pre-treatment, followed by incubated with HM-NPs@G for 24 h.
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J Adv Res
Selenoprotein H targets MTCH2 to regulate MFN2-dependent mitochondrial quality control to alleviate acute kidney injury. [Abstract]2025 Nov 26:S2090-1232(25)00955-5. PMID: 41314281 -
Biochem Pharmacol
Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability. [Abstract]2024 Nov 5;230(Pt 3):116611. PMID: 39510195
MFI8 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Nov 5;230(Pt 3):116611. [Abstract]
MFI8 (20 μM, 6 h). Representative immunoblot image of proteins associated with glutamate metabolism clearance.
MFI8 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2024 Nov 5;230(Pt 3):116611. [Abstract]
MFI8 (20 μM, 6 h). In the context of hyperglutamateemia (GV), H3R balances mitochondrial dynamics to restore the glutamate reuptake capacity of astrocytes.
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Radiother Oncol
Mitophagy induction improves salivary gland stem/progenitor cell function by reducing senescence after irradiation. [Abstract]2024 Jan:190:110028. PMID: 38007043
MFI8 purchased from MedChemExpress. Usage Cited in: Radiother Oncol. 2024 Jan:190:110028. [Abstract]
Representative images of whole-body staining of SA-β-galactosidase in salivary gland organoids irradiated (7 Gy) after treatment with MFI8 (20 μM) and DMSO.
MFI8 purchased from MedChemExpress. Usage Cited in: Radiother Oncol. 2024 Jan:190:110028. [Abstract]
Western blot analysis of p21 protein in salivary gland organoids irradiated with 7 Gy after treatment with MFI8 (20 μM) and DMSO.
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Exp Neurol
Biochanin A exerts neuroprotective effects in Parkinson's disease both in vivo and in vitro by improving mitochondrial dysfunction through the Sirt1 signaling pathway. [Abstract]2026 Apr:398:115636. PMID: 41483847 -
J Cell Mol Med
GCN5L1 Aggravates Postherpetic Neuralgia Through Regulating Microglial Mitochondrial Fission-Fusion Homeostasis. [Abstract]2025 Sep;29(18):e70861. PMID: 40988108 -
Neuroscience
Activin A protects against lipopolysaccharide/TNF-α induced damage of dopaminergic neurons both in vivo and in vitro by regulating mitochondrial fusion. [Abstract]2025 Sep 26:587:108-122. PMID: 41016503 -
Biochem Biophys Res Commun
Maladaptive role of peridroplet mitochondria during lipophagy disruption in pancreatic cancer. [Abstract]2025 Sep 30:782:152609. PMID: 40915065 -
Res Sq
TACO1 regulates mitochondrial adaptation in hypertension-induced cardiac remodeling and heart failure. [Abstract]2026 May 5:rs.3.rs-9589283. PMID: 42147162 -
Res Sq
A sodium-HIF1α axis coordinates immune metabolic reprogramming and mitochondrial remodeling in salt-sensitive hypertension. [Abstract]2026 Apr 24:rs.3.rs-9504540. PMID: 42078875 -
bioRxiv
Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction. [Abstract]2026 Feb 11:2026.02.10.704860. PMID: 41726991
Solvent & Solubility
DMSO : 100 mg/mL (362.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.07 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6262 mL | 18.1311 mL | 36.2621 mL | 90.6553 mL |
| 5 mM | 0.7252 mL | 3.6262 mL | 7.2524 mL | 18.1311 mL | |
| 10 mM | 0.3626 mL | 1.8131 mL | 3.6262 mL | 9.0655 mL | |
| 15 mM | 0.2417 mL | 1.2087 mL | 2.4175 mL | 6.0437 mL | |
| 20 mM | 0.1813 mL | 0.9066 mL | 1.8131 mL | 4.5328 mL | |
| 25 mM | 0.1450 mL | 0.7252 mL | 1.4505 mL | 3.6262 mL | |
| 30 mM | 0.1209 mL | 0.6044 mL | 1.2087 mL | 3.0218 mL | |
| 40 mM | 0.0907 mL | 0.4533 mL | 0.9066 mL | 2.2664 mL | |
| 50 mM | 0.0725 mL | 0.3626 mL | 0.7252 mL | 1.8131 mL | |
| 60 mM | 0.0604 mL | 0.3022 mL | 0.6044 mL | 1.5109 mL | |
| 80 mM | 0.0453 mL | 0.2266 mL | 0.4533 mL | 1.1332 mL | |
| 100 mM | 0.0363 mL | 0.1813 mL | 0.3626 mL | 0.9066 mL |
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