Mipasetamab
Based on 1 Customer Validation
Mipasetamab is an IgG1κ antibody targeting to AXL, a tyrosine kinase receptor and an TAM Receptor. Mipasetamab involves in synthesis of ADCT-601 (Mipasetamab uzoptirine), an AXL-targeted antibody-drug conjugate (ADC). ADCT-601 has anti-tumor activity.
For research use only. We do not sell to patients.
- Purity: 98.26%
- CAS No.: 2361055-48-1
- Molecular Weight:145.6 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Human IgG1 kappa
Human
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Axl |
ADCT-601 (0.02-9.29 nM; 5 d) shows cytotoxicity on AXL-positive SN12C cells but ont AXL-negative Karpas-299 cells[1].
ADCT-601 (0.83 nM; 2-36 h) increases the level of DNA interstrand cross-links time-dependently in SN12C cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:AXL-positive SN12C cells: Panc-1, A-172, SK-LU-1, MDA-MB-231, SK-OV-3, NCI-H1299, and SN12C
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Concentration:
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Incubation Time:5 days
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Result:Showed in vitro cytotoxicity with IC50s of 0.47 nM (Panc-1), 0.59 nM (A-172), 0.02 nM (SK-LU-1), 0.35 nM (MDA-MB-231), 0.11 nM (SK-OV-3), 2.2 nM (NCI-H1299), and 0.83 nM (SN12C), respectively.
ADCT-601 (1 mg/kg; i.v.; once daily for 60 days) shows potent and sustained antitumor activity for mouse in both MDA-MB-231 TNBC xenograft model and SN12C renal cancer xenograft model[1].
ADCT-601 (0.075, 0.15, or 0.3 mg/kg; i.v.; once daily for 45 days) also dose-dependently in a pancreatic cancer PDX model (PAXF1657) with heterogeneous AXL expression[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse model with SN12C renal cancer xenograft or MDA-MB-231 TNBC xenograft[1]
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Dosage:0.3, 0.6, and 1 mg/kg
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Administration:Intravenous injection; once daily for 60 days
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Result:Significantly inhibited the growth of tumor volume in vivo in mouse.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 kappa
ELISA, FACS, Functional assay
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Immobilized AXL Protein, Human can bind Mipasetamab. The EC50 for this effect is 29.13 ng/mL. -
Flow cytometric analysis of 1X106 Hela cells labeling Mipasetamab (HY-P99733, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa Isotype Control (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2361055-48-1
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Appearance Liquid
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Molecular Weight 145.6 kDa
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Color Colorless to light yellow
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SMILES
[Mipasetamab]
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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