mPEG750-amine  (Synonyms: mPEG750-NH2)

mPEG750-amine (mPEG750-NH2) is a chemical modification reagent for nanoparticles, capable of covalently binding to Ad-PVA to form Ad-PVA-PEG polymers. mPEG750-amine stabilizes gene delivery complexes by providing steric hindrance, reducing particle aggregation, while enhancing the water solubility and serum stability of the complex, reducing carrier cytotoxicity, and assisting in the efficient condensation of pDNA by cationic components to form nanoparticles that can be endocytosed by cells. mPEG750-amine can also be used to synthesize folate-conjugated polymer micelles for encapsulating the anticancer agent Camptothecin (HY-16560). Folate-conjugated polymer micelles are effective carriers for poorly soluble anticancer drugs, capable of avoiding macrophages and acting through folate receptor (FR)-mediated endocytosis to target tumor cells. mPEG750-amine can be applied to research in the field of non-viral gene delivery, as a component of gene delivery vectors, facilitating the safe and efficient delivery of nucleic acid drugs to target cells^[1][2].

Ad-PVA-PEG₇₅₀, synthesized from mPEG-amine (MW 750) via CDI activation reaction with Ad-PVA (stable for >5 days at pH=7, and 48 hours at pH=4), remains stable in a physiological environment at pH=7, but undergoes degradation via acetal bond cleavage under acidic conditions at pH=4, confirming its acid-responsive nature^[2].
Ad-PVA-PEG₇₅₀ synthesized from mPEG-amine (MW 750) (forming complexes with cationic β-CD derivatives and pDNA at an N:P ratio ≥5) effectively concentrates pDNA. Compared to cationic β-CD alone, complete pDNA condensation and inhibition of its migration in agarose gel can be achieved at a lower N:P ratio^[2].
The complex formed by Ad-PVA-PEG₇₅₀ synthesized from mPEG-amine (MW 750) and cationic β-CD exhibits 1000 times lower cytotoxicity to HeLa cells than 25 kDa bPEI, significantly reducing the cytotoxicity of the gene carrier^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

It can be used to evaluate the in vivo acute toxicity of mPEG-750 modified silicone nanoparticles, observing organ damage within 7-28 days^[1].
It can also be used to evaluate the effects of mPEG-750 modified silicone nanoparticles on the mouse hematopoietic system (hemolysis, coagulation function)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

750 (Average)

(C₂H₄O)_nC₃H₉NO

80506-64-5

Oil

Off-white to light yellow

COCCOCCN.[n]

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

• [1]. Jeong H, et al. In vitro blood cell viability profiling of polymers used in molecular assembly[J]. Scientific reports, 2017, 7(1): 9481.

  [2]. Kulkarni A, et al. Development of a low toxicity, effective pDNA vector based on noncovalent assembly of bioresponsive amino-β-cyclodextrin:adamantane-poly(vinyl alcohol)-poly(ethylene glycol) transfection complexes. Bioconjug Chem. 2012 May 16;23(5):933-40.  [Content Brief]