N-6-Methyl-2-deoxyadenosine (Synonyms: m6dA)

Name: N-6-Methyl-2-deoxyadenosine
Brand: MedChemExpress
SKU: HY-W011725
Rating: 4.5 (0 reviews)

Cat. No.: HY-W011725 Purity: 99.83%: Data Sheet
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N-6-Methyl-2-deoxyadenosine (m6dA) is an adenine nucleoside analogue. N-6-Methyl-2-deoxyadenosine targets nuclear processes and DNA replication machineries including WER, SATB1, TFAM, Jumu, SSBP1, DNA polymerase η and phage polymerase Gp90 exo⁻. N-6-Methyl-2-deoxyadenosine acts as a multifunctional epigenetic regulator that modulates transcription, DNA damage response, cell cycle, transposon silencing, stress adaptation, epigenetic crosstalk, and nucleosome organization in both prokaryotes and eukaryotes. N-6-Methyl-2-deoxyadenosine regulates mitochondrial epigenetic inheritance and is required for fear extinction memory in mice. N-6-Methyl-2-deoxyadenosine exhibits dysregulated levels in cancers. N-6-Methyl-2-deoxyadenosine can be used for the research of glioblastoma, triple negative breast cancer, and conditioned fear (fear extinction impairment).

For research use only. We do not sell to patients.

N-6-Methyl-2-deoxyadenosine Chemical Structure

CAS No. : 2002-35-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
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Other Forms of N-6-Methyl-2-deoxyadenosine:

N-6-Methyl-2-deoxyadenosine-d₃ In-stock

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Biological Activity
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References
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Description

IC₅₀ & Target^[1]

DNA Polymerase

In Vitro

N-6-Methyl-2-deoxyadenosine causes site-specific transcriptional pausing by Saccharomyces cerevisiae RNA polymerase II^[1].
N-6-Methyl-2-deoxyadenosine inhibits DNA replication by human DNA polymerase-η in vitro^[1].
N-6-Methyl-2-deoxyadenosine enhances the DNA-binding affinity of human mitochondrial SSBP1, reduces TFAM-mediated mtDNA transcription, and can be catalyzed and deposited onto mammalian mitochondrial DNA by human METTL4 in vitro^[1].
N-6-Methyl-2-deoxyadenosine reduces the binding affinity of Arabidopsis thaliana transcription factor WER to its target DNA motifs in vitro^[1].
N-6-Methyl-2-deoxyadenosine slightly increases the binding affinity of Drosophila melanogaster transcription factor Jumu to DNA in vitro^[1].
N-6-Methyl-2-deoxyadenosine inhibits DNA replication by Pseudomonas aeruginosa phage PaP1 DNA polymerase Gp90 exo⁻ ^[1].
N-6-Methyl-2-deoxyadenosine (20 mM; 7 h) was measured in mouse primary cortical neurons (7 days in vitro) treated with KCl, which exhibited a significant increase in global m6dA levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

N-6-Methyl-2-deoxyadenosine drives extinction-induced Bdnf exon IV expression in the mouse ILPFC and is required for the formation of fear extinction memory, with median freezing in extinction-trained N6amt1 knockdown mice reaching 49.68% compared to 10.57% in scrambled controls^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

265.27

Formula

C₁₁H₁₅N₅O₃

CAS No.

2002-35-9

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)C[C@H](N2C(N=CN=C3NC)=C3N=C2)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (376.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7697 mL	18.8487 mL	37.6974 mL
5 mM	0.7539 mL	3.7697 mL	7.5395 mL
10 mM	0.3770 mL	1.8849 mL	3.7697 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (279 KB) SDS (394 KB)

COA (254 KB) HNMR (122 KB) RP-HPLC (205 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Boulias K, et al. Means, mechanisms and consequences of adenine methylation in DNA. Nat Rev Genet. 2022;23(7):411-428. [Content Brief]

[2]. Li X, et al. The DNA modification N6-methyl-2'-deoxyadenosine (m6dA) drives activity-induced gene expression and is required for fear extinction. Nat Neurosci. 2019;22(4):534-544. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7697 mL	18.8487 mL	37.6974 mL	94.2436 mL
	5 mM	0.7539 mL	3.7697 mL	7.5395 mL	18.8487 mL
	10 mM	0.3770 mL	1.8849 mL	3.7697 mL	9.4244 mL
	15 mM	0.2513 mL	1.2566 mL	2.5132 mL	6.2829 mL
	20 mM	0.1885 mL	0.9424 mL	1.8849 mL	4.7122 mL
	25 mM	0.1508 mL	0.7539 mL	1.5079 mL	3.7697 mL
	30 mM	0.1257 mL	0.6283 mL	1.2566 mL	3.1415 mL
	40 mM	0.0942 mL	0.4712 mL	0.9424 mL	2.3561 mL
	50 mM	0.0754 mL	0.3770 mL	0.7539 mL	1.8849 mL
	60 mM	0.0628 mL	0.3141 mL	0.6283 mL	1.5707 mL
	80 mM	0.0471 mL	0.2356 mL	0.4712 mL	1.1780 mL
	100 mM	0.0377 mL	0.1885 mL	0.3770 mL	0.9424 mL

N-6-Methyl-2-deoxyadenosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-6-Methyl-2-deoxyadenosine2002-35-9m6dANucleoside Antimetabolite/AnalogMitochondrial MetabolismDNA/RNA SynthesisJumutriple negative breast cancerSaccharomyces cerevisiae RNA polymerase IITFAMhuman DNA polymerase-ηSATB1glioblastomaSSBP1WERCaenorhabditis elegansInhibitorinhibitorinhibit

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N-6-Methyl-2-deoxyadenosine (Synonyms: m6dA)

Customer Review

Other Forms of N-6-Methyl-2-deoxyadenosine:

N-6-Methyl-2-deoxyadenosine Related Antibodies

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•Related Screening Libraries:

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Biological Activity

Purity & Documentation

References

Customer Review

N-6-Methyl-2-deoxyadenosine Related Antibodies

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Complete Stock Solution Preparation Table

N-6-Methyl-2-deoxyadenosine Related Classifications

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