N-Benzylaniline
Based on 1 Customer Validation
N-Benzylaniline (N-Phenylbenzylamine) is an N-alkylated derivative of aniline.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 103-32-2
- Formula: C13H13N
- Molecular Weight:183.25
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
N-Benzylaniline in the acidic medium coupled with diazotized dapsone to form the azo dye and can be used to determination of nitrite in water and aqueous solutions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 103-32-2
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Appearance <35°C Solid,>38°C Liquid
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Molecular Weight 183.25
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Formula C13H13N
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Color Light yellow to brown
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SMILES
C1(CNC2=CC=CC=C2)=CC=CC=C1
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Synonyms
N-Phenylbenzylamine; Benzylaniline
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (545.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4569 mL | 27.2845 mL | 54.5691 mL | 136.4227 mL |
| 5 mM | 1.0914 mL | 5.4569 mL | 10.9138 mL | 27.2845 mL | |
| 10 mM | 0.5457 mL | 2.7285 mL | 5.4569 mL | 13.6423 mL | |
| 15 mM | 0.3638 mL | 1.8190 mL | 3.6379 mL | 9.0948 mL | |
| 20 mM | 0.2728 mL | 1.3642 mL | 2.7285 mL | 6.8211 mL | |
| 25 mM | 0.2183 mL | 1.0914 mL | 2.1828 mL | 5.4569 mL | |
| 30 mM | 0.1819 mL | 0.9095 mL | 1.8190 mL | 4.5474 mL | |
| 40 mM | 0.1364 mL | 0.6821 mL | 1.3642 mL | 3.4106 mL | |
| 50 mM | 0.1091 mL | 0.5457 mL | 1.0914 mL | 2.7285 mL | |
| 60 mM | 0.0909 mL | 0.4547 mL | 0.9095 mL | 2.2737 mL | |
| 80 mM | 0.0682 mL | 0.3411 mL | 0.6821 mL | 1.7053 mL | |
| 100 mM | 0.0546 mL | 0.2728 mL | 0.5457 mL | 1.3642 mL |