N-Deacetylcolchicine

Cat. No.: HY-121434 Purity: 98.38%: Data Sheet
COA

SDS
Handling Instructions
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N-Deacetylcolchicine is a microtubule polymerization inhibitor with an IC₅₀ of 3 μM against bovine brain microtubules. N-Deacetylcolchicine is a derivative of Colchicine (HY-16569). N-Deacetylcolchicine can activate the GTPase activity of microtubules and can be used for the research of cancer.

For research use only. We do not sell to patients.

N-Deacetylcolchicine Chemical Structure

CAS No. : 3476-50-4

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Other Forms of N-Deacetylcolchicine:

N-Deacetylcolchicine tartrate Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.036 μM Compound: 15	Cytotoxicity against human A549 cells after 96 hrs using tetracolor one Cytotoxicity against human A549 cells after 96 hrs using tetracolor one	[PMID: 24900316]
H9	EC₅₀	0.2 μM Compound: 2	Cytotoxicity against human H9 cells Cytotoxicity against human H9 cells	[PMID: 1919593]
H9	IC₅₀	0.2 μM Compound: 2	Antiviral activity against HIV1 HTLV-3B in human H9 cells after 3 days by p24 antigen capture assay Antiviral activity against HIV1 HTLV-3B in human H9 cells after 3 days by p24 antigen capture assay	[PMID: 1919593]
HBL-100	IC₅₀	0.045 μM Compound: 2	Antiproliferative activity against human HBL100 after 72 hrs by MTT assay Antiproliferative activity against human HBL100 after 72 hrs by MTT assay	[PMID: 22739088]
HT-29	IC₅₀	0.025 μM Compound: 15	Cytotoxicity against human HT-29 cells after 96 hrs using tetracolor one Cytotoxicity against human HT-29 cells after 96 hrs using tetracolor one	[PMID: 24900316]
HUVEC	IC₅₀	0.034 μM Compound: 15	Cytotoxicity against human HUVEC cells after 96 hrs using tetracolor one Cytotoxicity against human HUVEC cells after 96 hrs using tetracolor one	[PMID: 24900316]

Molecular Weight

357.40

Formula

C₂₀H₂₃NO₅

CAS No.

3476-50-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

COC1=C2C(C([C@H](CCC2=CC(OC)=C1OC)N)=C3)=CC=C(OC)C3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (27.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7980 mL	13.9899 mL	27.9799 mL
5 mM	0.5596 mL	2.7980 mL	5.5960 mL
10 mM	0.2798 mL	1.3990 mL	2.7980 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.38%

Select Batch:

Data Sheet (279 KB) SDS (396 KB)

COA (260 KB) HNMR (258 KB) RP-HPLC (230 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Muzaffar, A., et al. Antitubulin effects of derivatives of 3-demethylthiocolchicine, methylthio ethers of natural colchicinoids, and thioketones derived from thiocolchicine. Comparison with colchicinoids. J. Med. Chem. 33(2), 567-571 (1990). [Content Brief]

[2]. Lin, et al. Effects of inhibitors of tubulin polymerization on GTP hydrolysis. The Journal of Biological Chemisty 256(17), 9242-9245 (1981). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7980 mL	13.9899 mL	27.9799 mL	69.9496 mL
	5 mM	0.5596 mL	2.7980 mL	5.5960 mL	13.9899 mL
	10 mM	0.2798 mL	1.3990 mL	2.7980 mL	6.9950 mL
	15 mM	0.1865 mL	0.9327 mL	1.8653 mL	4.6633 mL
	20 mM	0.1399 mL	0.6995 mL	1.3990 mL	3.4975 mL
	25 mM	0.1119 mL	0.5596 mL	1.1192 mL	2.7980 mL