N-Nonyldeoxynojirimycin
Based on 1 Customer Validation
N-Nonyldeoxynojirimycin (NN-DNJ) is a deoxynojirimycin derivative with a hydrophobic long chain. N-Nonyldeoxynojirimycin is an orally active inhibitor of acid α-glucosidase (IC50: 0.42 μM) and α-1,6-glucosidase (IC50: 8.4 μM). N-Nonyldeoxynojirimycin has activities of inhibiting glycogen breakdown, anti-virus and anti-tumor. N-Nonyldeoxynojirimycin also has insecticidal effects, with an LD50 of 5 mM for adults of Bemisia tabaci.
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- Purity : 98.0%
- CAS No.: 81117-35-3
- 화학식: C15H31NO4
- 분자량:289.41
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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All Parasite Isoforms
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Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| RAW | IC50 |
4 μM
Compound: 9
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Inhibition of glucosylceramide synthase in mouse RAW cells preincubated with compound for 15 mins by in-situ enzyme inhibition assay
Inhibition of glucosylceramide synthase in mouse RAW cells preincubated with compound for 15 mins by in-situ enzyme inhibition assay
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[PMID: 24900289] |
| SH-SY5Y | IC50 |
0.003 μM
Compound: 2; D-NNDNJ
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Inhibition of NLGase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay
Inhibition of NLGase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay
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[PMID: 31075609] |
| SH-SY5Y | IC50 |
5.6 μM
Compound: 2; D-NNDNJ
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Inhibition of GCase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of conduritol B epoxide by fluorescence based assay
Inhibition of GCase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of conduritol B epoxide by fluorescence based assay
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[PMID: 31075609] |
| Vero | CC50 |
>40 μM
Compound: NNDNJ
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Cytotoxicity against african green monkey Vero cells by MTT assay
Cytotoxicity against african green monkey Vero cells by MTT assay
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[PMID: 19223639] |
| Vero | EC50 |
1.1 μM
Compound: NNDNJ
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Antiviral activity against Dengue virus C infected in african green monkey Vero cells assessed as reduction in viral titer after 72 hrs by plaque reduction assay
Antiviral activity against Dengue virus C infected in african green monkey Vero cells assessed as reduction in viral titer after 72 hrs by plaque reduction assay
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[PMID: 19223639] |
In Vitro
N-Nonyldeoxynojirimycin (0-200 μM; 3 days) inhibits the secretion of infectious bovine viral diarrhea virus (BVDV) in MDBK cells with an IC50 of 2.5 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
N-Nonyldeoxynojirimycin (dietary administration) reduces tumor growth and ganglioside content with fewer side effects compared to Miglustat (HY-17020) in C57BL/6 mice bearing CT-2A tumors[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female C57BL/6 mice[2]
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Dosage:250 mg/kg/day for 1-120 days or 10-250 mg/kg/day for 30 days
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Administration:Dietary administration
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Result:Significantly increased liver glycogen concentration to 526 mmol/g wet weight in fasted mice at 250 mg/kg/day for 30 days, with effects plateauing at 50 days and persisting for 120 days. Glycogen accumulation was also observed in gastrocnemius muscle of both fed and fasted mice, and occasional glycogen deposition was seen in heart sections.
Induced dose-dependent liver glycogen inhibition at lower doses (≥50 mg/kg/day), with significant effects at 50 mg/kg/day .
Chemical Information
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CAS No. 81117-35-3
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Appearance Solid
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분자량 289.41
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화학식 C15H31NO4
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Color White to off-white
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Synonyms
NN-DNJ; Nonyl-DNJ
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 50 mg/mL (172.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Acute Systemic Toxicity Study
Acute systemic toxicity studies evaluate adverse effects occurring after a single exposure, or repeated exposure within a short acute window, and the main in vivo readouts are mortality, moribund condition, clinical signs, body-weight change, and gross pathological findings; acute oral toxicity methods were developed to replace classical LD50 testing with reduced-animal designs such as fixed-dose procedure, acute toxic class method, and up-and-down procedure. The fixed-dose procedure classifies acute toxicity by administering predefined dose levels and observing evident toxicity rather than using death as the primary endpoint, whereas the acute toxic class method uses sequential groups of three animals per step and the up-and-down procedure doses animals sequentially to estimate an LD50 with fewer animals than conventional LD50 testing.
순도&문서
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zitzmann N, et al. Imino sugars inhibit the formation and secretion of bovine viral diarrhea virus, a pestivirus model of hepatitis C virus: implications for the development of broad spectrum anti-hepatitis virus agents. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11878-82. [Content Brief]
[2]. Andersson U, et al. Inhibition of glycogen breakdown by imino sugars in vitro and in vivo. Biochem Pharmacol. 2004 Feb 15;67(4):697-705. [Content Brief]
[4]. Ya'kobovitz MK, et al. Inhibition of α-glucosidase activity by N-deoxynojirimycin analogs in several insect phloem sap feeders. Insect Sci. 2016 Feb;23(1):59-67. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4553 mL | 17.2765 mL | 34.5531 mL | 86.3826 mL |
| 5 mM | 0.6911 mL | 3.4553 mL | 6.9106 mL | 17.2765 mL | |
| 10 mM | 0.3455 mL | 1.7277 mL | 3.4553 mL | 8.6383 mL | |
| 15 mM | 0.2304 mL | 1.1518 mL | 2.3035 mL | 5.7588 mL | |
| 20 mM | 0.1728 mL | 0.8638 mL | 1.7277 mL | 4.3191 mL | |
| 25 mM | 0.1382 mL | 0.6911 mL | 1.3821 mL | 3.4553 mL | |
| 30 mM | 0.1152 mL | 0.5759 mL | 1.1518 mL | 2.8794 mL | |
| 40 mM | 0.0864 mL | 0.4319 mL | 0.8638 mL | 2.1596 mL | |
| 50 mM | 0.0691 mL | 0.3455 mL | 0.6911 mL | 1.7277 mL | |
| 60 mM | 0.0576 mL | 0.2879 mL | 0.5759 mL | 1.4397 mL | |
| 80 mM | 0.0432 mL | 0.2160 mL | 0.4319 mL | 1.0798 mL | |
| 100 mM | 0.0346 mL | 0.1728 mL | 0.3455 mL | 0.8638 mL |
N-Nonyldeoxynojirimycin Related Classifications
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