NAE-IN-1
Based on 1 Customer Validation
NAE-IN-1 (compound X-10) is a potent NAE1 inhibitor. NAE-IN-1 induces apoptosis and cell cycle arrest at the G2/M phase. NAE-IN-1 increases ROS levels and prevents cell migration. NAE-IN-1 shows anti-proliferation activity.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 3058629-66-3
- Formula: C29H30N4O2S
- Molecular Weight:498.64
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Metabolism/Protease Compound Library
HY-L012
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NF-κB Signaling Compound Library
HY-L014
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Ubiquitination Compound Library
HY-L050
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Metabolic Enzyme Compound Library
HY-L146
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
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Lactylation Compound Library
HY-L249
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.87 μM
Compound: X-10
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Antiproliferative activity against human A549 cells by CCK8 assay
Antiproliferative activity against human A549 cells by CCK8 assay
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[PMID: 38320715] |
| HUVEC | IC50 |
6.21 μM
Compound: X-10
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Cytotoxicity against HUVECs incubated for 72 hrs by CCK8 assay
Cytotoxicity against HUVECs incubated for 72 hrs by CCK8 assay
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[PMID: 38320715] |
| KYSE-30 | IC50 |
0.65 μM
Compound: X-10
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Antiproliferative activity against human KYSE-30 cells by CCK8 assay
Antiproliferative activity against human KYSE-30 cells by CCK8 assay
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[PMID: 38320715] |
| MCF7 | IC50 |
0.96 μM
Compound: X-10
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Antiproliferative activity against human MCF7 cells by CCK8 assay
Antiproliferative activity against human MCF7 cells by CCK8 assay
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[PMID: 38320715] |
| MGC-803 | IC50 |
1.63 μM
Compound: X-10
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Antiproliferative activity against human MGC-803 cells by CCK8 assay
Antiproliferative activity against human MGC-803 cells by CCK8 assay
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[PMID: 38320715] |
NAE-IN-1 (compound X-10) (1, 3, 5 μM; 24 h) decreases the expression of Cullin 1 and Cullin 3 in a dose-dependent manner in MGC-803 cells[1].
NAE-IN-1 (0, 1, 2, 4 μM; 24 h) induces cell cycle arrest at G2/M phase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549, MGC-803, MCF-7, KYSE-30 cells
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Concentration:0-100 µM
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Incubation Time:
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Result:Showed anti-proliferation activity with IC50s of 0.87, 1.63, 0.96, 0.65 µM for A549, MGC-803, MCF-7, KYSE-30 cells, respectively.
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Cell Line:MGC-803 cells
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Concentration:1, 3, 5 µM
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Incubation Time:24 h
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Result:Decreased the expression of Cullin 1 and Cullin 3 in a dose-dependent manner.
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Cell Line:MGC-803 cells
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Concentration:0, 1, 2, 4 µM
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Incubation Time:24 h
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Result:Induced cell cycle arrest at G2/M phase.
Chemical Information
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CAS No. 3058629-66-3
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Appearance Solid
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Molecular Weight 498.64
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Formula C29H30N4O2S
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Color White to off-white
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SMILES
O=C(NC1=CC=C(N=C(NC(C2=CC=C(CC)C=C2)=O)S3)C3=C1)C(CC4)CCN4CC5=CC=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (200.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.03 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0055 mL | 10.0273 mL | 20.0545 mL | 50.1364 mL |
| 5 mM | 0.4011 mL | 2.0055 mL | 4.0109 mL | 10.0273 mL | |
| 10 mM | 0.2005 mL | 1.0027 mL | 2.0055 mL | 5.0136 mL | |
| 15 mM | 0.1337 mL | 0.6685 mL | 1.3370 mL | 3.3424 mL | |
| 20 mM | 0.1003 mL | 0.5014 mL | 1.0027 mL | 2.5068 mL | |
| 25 mM | 0.0802 mL | 0.4011 mL | 0.8022 mL | 2.0055 mL | |
| 30 mM | 0.0668 mL | 0.3342 mL | 0.6685 mL | 1.6712 mL | |
| 40 mM | 0.0501 mL | 0.2507 mL | 0.5014 mL | 1.2534 mL | |
| 50 mM | 0.0401 mL | 0.2005 mL | 0.4011 mL | 1.0027 mL | |
| 60 mM | 0.0334 mL | 0.1671 mL | 0.3342 mL | 0.8356 mL | |
| 80 mM | 0.0251 mL | 0.1253 mL | 0.2507 mL | 0.6267 mL | |
| 100 mM | 0.0201 mL | 0.1003 mL | 0.2005 mL | 0.5014 mL |
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