1. Apoptosis
  2. RIP kinase
  3. Necrostatin-34

Necrostatin-34 

Cat. No.: HY-132203
Handling Instructions

Necrostatin-34 (Nec-34), a RIPK1 kinase inhibitor, stabilizes RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain.

For research use only. We do not sell to patients.

Necrostatin-34 Chemical Structure

Necrostatin-34 Chemical Structure

CAS No. : 375835-43-1

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Description

Necrostatin-34 (Nec-34), a RIPK1 kinase inhibitor, stabilizes RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain[1].

In Vitro

Necrostatin-34 (Nec-34) exhibits IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively[1].
Necrostatin-34 (Nec-34, 10 μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation[1].
Necrostatin-34 (Nec-34) may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: RIPK1 knockout L929 cells transfected with an expressing vector encoding an inducible and dimerizable RIPK1 fused with FKBP at the C-terminus.
Concentration: 10 μM.
Incubation Time: 30 min (and then 100 ng/mL TNFα was added for indicated periods of time).
Result: Downregulated p-S166 levels.
Molecular Weight

384.48

Formula

C₁₈H₁₆N₄O₂S₂

CAS No.
SMILES

O=C(NC1=NC=CS1)CSC2=C(C#N)C(C3=CC=C(C)C=C3)CC(N2)=O

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Keywords:

Necrostatin-34Necrostatin34Necrostatin 34RIP kinaseReceptor-interacting protein kinasesRIPKNecroptosisp-S166RIPK1FADDInhibitorinhibitorinhibit

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