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Cat. No.: HY-187042

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Nirtetralin

Nirtetralin is a lignan. Nirtetralin can be isolated from Phyllanthus urinaria, Phyllanthus virgatus Forst. f., and Phyllanthus niruri L. Nirtetralin undergoes metabolic activation to generate active intermediates, such as nitroalkene ions and nitroso compounds, which can form DNA adducts and induce DNA damage. Nirtetralin induces ROS production in cells. Nirtetralin blocks the epithelial-mesenchymal transition process of cancer cells by altering the expression of related markers. Nirtetralin inhibits the production of HBsAg and HBeAg. Nirtetralin exhibits anticancer activity against oral squamous cell carcinoma. Nirtetralin can be used in studies of oral squamous cell carcinoma and hepatitis B virus infection.

For research use only. We do not sell to patients.

CAS No.: 50656-78-5
Formula: C₂₄H₃₀O₇
Molecular Weight:430.50

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Nirtetralin Chemical Structure

CAS No.: 50656-78-5

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
KB	IC50	159.15 μM	Antiproliferative activity against human KB oral squamous carcinoma cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay. Antiproliferative activity against human KB oral squamous carcinoma cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay.	40353242
KB	IC50	75.89 μM	Antiproliferative activity against human KB oral squamous carcinoma cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay. Antiproliferative activity against human KB oral squamous carcinoma cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay.	40353242
MS-G2	EC50	36.9 μM	Suppression of HBsAg production in HBV-producing MS-G2 cells assessed via ELISA after 3 days of incubation. Suppression of HBsAg production in HBV-producing MS-G2 cells assessed via ELISA after 3 days of incubation.	12748977

In Vitro

Nirtetralin (10-120 μM; 24-48 h) inhibits the viability of KB oral squamous carcinoma cells in a dose- and time-dependent manner, with an IC₅₀ of 159.15 μM at 24 hours and 75.89 μM at 48 hours^[1].
Nirtetralin modulates epithelial-mesenchymal transition (EMT) in KB oral squamous carcinoma cells by upregulating CLD1 and downregulating ECADH, VIM, SNAIL1, and SLUG gene expression^[1].
Nirtetralin (5-50 μM) non-cytotoxically suppresses HBsAg and HBeAg production in MS-G2 cells, with an EC₅₀ of 36.9 μM for HBsAg inhibition and maximum inhibitions of 69.6% for HBsAg and 33.9% for HBeAg at 50 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

KB oral squamous carcinoma cells
Concentration:

10-120 μM (24 h incubation); 10-120 μM (48 h incubation)
Incubation Time:

24 h; 48 h
Result:

Caused a significant, dose-dependent decrease in KB cell viability, with a more pronounced cytotoxic effect after 48 hours compared to 24 hours.
Exhibited a half-maximal inhibitory concentration (IC₅₀) of 159.15 μM after 24 hours of incubation.
Exhibited a half-maximal inhibitory concentration (IC₅₀) of 75.89 μM after 48 hours of incubation.

ELISA Assay^[2]

Cell Line:

MS-G2 cells
Concentration:

5-50 μM
Incubation Time:

Unclear
Result:

Suppressed HBsAg production by 69.6% at 50 μM.
Suppressed HBeAg production by 33.9% at 50 μM.
Suppressed HBsAg production by 32.1% at 25 μM.
Suppressed HBeAg production by 23.7% at 25 μM.
Suppressed HBsAg production by 22.4% at 5 μM.
Exhibited no significant effect on HBeAg production at 5 μM.
Achieved an EC₅₀ of 36.9 μM for HBsAg suppression.

Chemical Information

CAS No. 50656-78-5
Molecular Weight 430.50
Formula C₂₄H₃₀O₇
SMILES

O(C)C=1C=2[C@H]([C@H](COC)[C@@H](COC)CC2C=C3C1OCO3)C4=CC(OC)=C(OC)C=C4
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Murugan R, et al. Interpreting nirtetralin's anticancer efficacy in oral squamous cell carcinoma: Integrating experimental and computational perspectives. Toxicology reports. 2025 Jun;14:102034.

[2]. Huang RL, et al. Screening of 25 compounds isolated from Phyllanthus species for anti-human hepatitis B virus in vitro. Phytotherapy research : PTR. 2003 May;17(5):449-53.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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