Ophiobolin A
Based on 1 Customer Validation
Ophiobolin A, a fungal metabolite and a phytotoxin, is a potent and irreversibly inhibitor of calmodulin-activated cyclic nucleotide phosphodiesterase, with an IC50 value of 9 μM. Ophiobolin A antimicrobial and anticancer activity.
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- Pureté : 98.86%
- CAS No.: 4611-05-6
- Formule: C25H36O4
- Masse moléculaire:400.55
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
IC50: 9 μM (calmodulin-activated cyclic nucleotide phosphodiesterase)[1].
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | GI50 |
0.1 μM
Compound: Ophiobolin A
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Antiproliferative activity against human A549 cells by MTT assay
Antiproliferative activity against human A549 cells by MTT assay
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[PMID: 26341136] |
| A549 | IC50 |
50 ng/mL
Compound: 2
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Cytotoxicity against human A549 cells
Cytotoxicity against human A549 cells
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[PMID: 10395513] |
| B16-F10 | IC50 |
0.29 μM
Compound: 3
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Antiproliferative activity against mouse B16-F10 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Antiproliferative activity against mouse B16-F10 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 33074690] |
| Hs 683 | GI50 |
0.02 μM
Compound: Ophiobolin A
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Antiproliferative activity against human Hs683 cells by MTT assay
Antiproliferative activity against human Hs683 cells by MTT assay
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[PMID: 26341136] |
| HT-29 | IC50 |
50 ng/mL
Compound: 2
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Cytotoxicity against human HT-29 cells
Cytotoxicity against human HT-29 cells
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[PMID: 10395513] |
| MCF7 | GI50 |
0.06 μM
Compound: Ophiobolin A
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Antiproliferative activity against human MCF7 cells by MTT assay
Antiproliferative activity against human MCF7 cells by MTT assay
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[PMID: 26341136] |
| P388 | IC50 |
25 ng/mL
Compound: 2
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 10395513] |
| Panel NCI-60 (60 carcinoma cell lines) | GI50 |
72 nM
Compound: 1
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Cytotoxicity against human NCI60 cells
Cytotoxicity against human NCI60 cells
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[PMID: 30765189] |
| SK-MEL-28 | GI50 |
0.05 μM
Compound: Ophiobolin A
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Antiproliferative activity against human SK-MEL-28 cells by MTT assay
Antiproliferative activity against human SK-MEL-28 cells by MTT assay
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[PMID: 26341136] |
| U-373MG ATCC | GI50 |
0.06 μM
Compound: Ophiobolin A
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Antiproliferative activity against human U373 cells by MTT assay
Antiproliferative activity against human U373 cells by MTT assay
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[PMID: 26341136] |
Chemical Information
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CAS No. 4611-05-6
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Appearance Solid
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Masse moléculaire 400.55
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Formule C25H36O4
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Color White to off-white
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SMILES
O=C/C1=C/C[C@]2([H])[C@@](CC[C@@]23O[C@@H](/C=C(C)\C)C[C@@H]3C)(C)C[C@@]4([H])[C@]1([H])C(C[C@]4(O)C)=O
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Structure Classification
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Initial Source
fungus Bipolaris sp.
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
Methanol : 4 mg/mL (9.99 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol | 1 mM | 2.4966 mL | 12.4828 mL | 24.9657 mL | 62.4142 mL |
| 5 mM | 0.4993 mL | 2.4966 mL | 4.9931 mL | 12.4828 mL |
Ophiobolin A Related Classifications
HY-N6781 Related Classifications
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