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Cat. No.: HY-175819

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PD-166285

PD-166285 is a PKMYT1 inhibitor, with an IC₅₀ value of 17 nM. PD166285 shows strong antiproliferative effects against CCNE1-amplified OVCAR3 cells (IC₅₀ = 0.14 μM) and HCC1569 cells (IC₅₀ = 0.21 μM). PD166285 induces apoptosis and arrests CCNE1-amplified HCC1569 cells at the G1/S phase of the cell cycle. PD166285 can be used for the research of PKMYT1-targeted therapies for CCNE1-amplified cancers.

For research use only. We do not sell to patients.

CAS No.: 717094-37-6
Formula: C₂₄H₂₃Cl₂N₅O₂
Molecular Weight:484.38

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

PD-166285 Chemical Structure

CAS No.: 717094-37-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PKMYT1

17 nM (IC₅₀)

All Wee1 Isoforms :

PKMYT1

In Vitro

PD166285 (0.062-1 μM, 5 days) shows strong antiproliferative effects against CCNE1-amplified OVCAR3 cells (IC₅₀ = 0.14 μM) and HCC1569 cells (IC₅₀ = 0.21 μM)^[1].
PD166285 (14 days) inhibits colony formation of CCNE1-amplified OVCAR3 cells in a concentration-dependent manner (EC₅₀ = 0.06 μM) and HCC1569 cells (EC₅₀ = 0.16 μM)^[1].
PD166285 (0.125-1 μM, 5 days) induces apoptosis and arrests CCNE1-amplified HCC1569 cells at the G1/S phase of the cell cycle^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

CCNE1-amplified HCC1569 cells
Concentration:

0.125, 0.25, 0.5 μM
Incubation Time:

5 days
Result:

Arrested CCNE1-amplified HCC1569 cells at the G1/S phase of the cell cycle.

Apoptosis Analysis^[1]

Cell Line:

CCNE1-amplified HCC1569 cells
Concentration:

0.125, 0.25, 0.5, 1 μM
Incubation Time:

5 days
Result:

Induced apoptosis in CCNE1-amplified HCC1569 cells.

Chemical Information

CAS No. 717094-37-6
Molecular Weight 484.38
Formula C₂₄H₂₃Cl₂N₅O₂
SMILES

ClC1=CC=CC(Cl)=C1C2=CC3=C(N=C(NC4=CC=C(OCCN(C)C)C=C4)N=C3)N(C)C2=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang H, et al. Discovery of potent and selective PKMYT1 inhibitors for the treatment of CCNE1-amplified cancer. Eur J Med Chem. 2025 Dec 5;299:118059. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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