1. Epigenetics PI3K/Akt/mTOR
  2. AMPK
  3. PF-06685249

PF-06685249  (Synonyms: PF-249)

Cat. No.: HY-117623 Purity: 98.32%
COA Handling Instructions

PF-06685249 (PF-249) is a potent and orally active allosteric AMPK activator with an EC50 of 12 nM for recombinant AMPK α1β1γ1. PF-06685249 can be used for diabetic nephropathy research.

For research use only. We do not sell to patients.

PF-06685249 Chemical Structure

PF-06685249 Chemical Structure

CAS No. : 1467059-70-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 286 In-stock
Solution
10 mM * 1 mL in DMSO USD 286 In-stock
Solid
5 mg USD 260 In-stock
10 mg USD 380 In-stock
25 mg USD 800 In-stock
50 mg USD 1280 In-stock
100 mg USD 2100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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Description

PF-06685249 (PF-249) is a potent and orally active allosteric AMPK activator with an EC50 of 12 nM for recombinant AMPK α1β1γ1. PF-06685249 can be used for diabetic nephropathy research[1].

IC50 & Target[1]

AMPK α1β1γ1

12 nM (EC50)

Molecular Weight

345.78

Formula

C17H16ClN3O3

CAS No.
Appearance

Solid

Color

Off-white to gray

SMILES

O=C(C1=CNC2=C1C=C(C3=CC=C(N(C)C)N=C3OC)C(Cl)=C2)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (289.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8920 mL 14.4601 mL 28.9201 mL
5 mM 0.5784 mL 2.8920 mL 5.7840 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8920 mL 14.4601 mL 28.9201 mL 72.3003 mL
5 mM 0.5784 mL 2.8920 mL 5.7840 mL 14.4601 mL
10 mM 0.2892 mL 1.4460 mL 2.8920 mL 7.2300 mL
15 mM 0.1928 mL 0.9640 mL 1.9280 mL 4.8200 mL
20 mM 0.1446 mL 0.7230 mL 1.4460 mL 3.6150 mL
25 mM 0.1157 mL 0.5784 mL 1.1568 mL 2.8920 mL
30 mM 0.0964 mL 0.4820 mL 0.9640 mL 2.4100 mL
40 mM 0.0723 mL 0.3615 mL 0.7230 mL 1.8075 mL
50 mM 0.0578 mL 0.2892 mL 0.5784 mL 1.4460 mL
60 mM 0.0482 mL 0.2410 mL 0.4820 mL 1.2050 mL
80 mM 0.0362 mL 0.1808 mL 0.3615 mL 0.9038 mL
100 mM 0.0289 mL 0.1446 mL 0.2892 mL 0.7230 mL
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PF-06685249 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
PF-06685249
Cat. No.:
HY-117623
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