2. Cell Cycle/DNA Damage
  3. Endonuclease
  4. PFM01

PFM01, N-alkylated Mirin derivative, is a MRE11 endonuclease inhibitor. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR).

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PFM01 Chemical Structure

PFM01 Chemical Structure

CAS No. : 1558598-41-6

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

PFM01 Related Antibodies

Top Publications Citing Use of Products

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Description

PFM01, N-alkylated Mirin derivative, is a MRE11 endonuclease inhibitor. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR)[1][2].

IC50 & Target

Endonuclease[1]
In Vitro

PFM01 (100 μM) rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells[1].
PFM01 (100 μM) diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells[1].
PFM01 (100 μM) enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells[1].
PFM01 substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PFM01 Related Antibodies
Molecular Weight

293.40

Formula

C14H15NO2S2
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1N(CC(C)C)C(S/C1=C\C2=CC=C(O)C=C2)=S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (340.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (85.21 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4083 mL 17.0416 mL 34.0832 mL
5 mM 0.6817 mL 3.4083 mL 6.8166 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.4083 mL 17.0416 mL 34.0832 mL 85.2079 mL
5 mM 0.6817 mL 3.4083 mL 6.8166 mL 17.0416 mL
10 mM 0.3408 mL 1.7042 mL 3.4083 mL 8.5208 mL
15 mM 0.2272 mL 1.1361 mL 2.2722 mL 5.6805 mL
20 mM 0.1704 mL 0.8521 mL 1.7042 mL 4.2604 mL
25 mM 0.1363 mL 0.6817 mL 1.3633 mL 3.4083 mL
30 mM 0.1136 mL 0.5681 mL 1.1361 mL 2.8403 mL
40 mM 0.0852 mL 0.4260 mL 0.8521 mL 2.1302 mL
50 mM 0.0682 mL 0.3408 mL 0.6817 mL 1.7042 mL
60 mM 0.0568 mL 0.2840 mL 0.5681 mL 1.4201 mL
80 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0651 mL
DMSO 100 mM 0.0341 mL 0.1704 mL 0.3408 mL 0.8521 mL
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PFM01 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PFM011558598-41-6PFM 01PFM-01EndonucleaseN-alkylatedMirinderivativeMRE11endonucleaseNHEJDSBInhibitorinhibitorinhibit

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