Phytolacca americana Lectin
Phytolacca americana Lectin (PWM) is an acidic monomeric glycoprotein specific to N-acetylglucosamine and a B cell-specific stimulator. Phytolacca americana Lectin acts as a mitogen to induce cell mitosis. Phytolacca americana Lectin sensitizes B cells to Toll-like receptor stimulation, enabling their activation by immunostimulatory DNA. When used in combination with copurified microbial Toll-like receptor ligands, Phytolacca americana Lectin triggers B cell proliferation and immunoglobulin secretion.
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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IL-8 |
Phytolacca americana Lectin induces mitosis in peripheral lymphocytes through cell surface binding[2].
Phytolacca americana Lectin (1-10 μg/mL) induces the proliferation of live-gated CD19+ peripheral blood B cells and promotes plasmablast differentiation, while B cell receptor cross-linking via anti-Ig interferes with these effector functions[3].
Phytolacca americana Lectin significantly induces the expression of intracellular TNF in monocytes, as detected by mean fluorescence intensity[3].
Phytolacca americana Lectin (10 μg/mL; 24 h) induces IL-8 secretion in HEK293 cells transfected with TLR2, and this activity is partially dependent on TLR2[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human TLR2-transfected HEK293 cells
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Concentration:10 μg/mL
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Incubation Time:24 h
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Result:Induced IL-8 secretion in TLR2-transfected HEK293 cells, with production almost doubled compared to non-transfected cells.
Reduced PWM-induced IL-8 production by approximately 50% (p=0.03) compared to isotype control after pre-treatment with 10 μg/mL anti-TLR2 mAb.
Chemical Information
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SMILES
[Phytolacca americana Lectin]
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Synonyms
PWM
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Ahmad E, et al. Phytolacca americana lectin (Pa-2; pokeweed mitogen): an intrinsically unordered protein and its conversion into partial order at low pH. Biosci Rep. 2009 Nov 23;30(2):125-34. [Content Brief]
[2]. Fujii T, et al. Structures of two lectins from the roots of pokeweed (Phytolacca americana). Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):665-73. [Content Brief]
[3]. Bekeredjian-Ding I, et al. Poke weed mitogen requires Toll-like receptor ligands for proliferative activity in human and murine B lymphocytes. PloS one. 2012;7(1):e29806. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phytolacca americana Lectin Related Classifications
HY-NP187 Related Classifications
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