(+)-Intermedine
Based on 1 publication(s) in Google Scholar
(+)-Intermedine, a pyrrolizidine alkaloid (PA), exhibits significant cytotoxicity in neural progenitor cells (NPCs).
For research use only. We do not sell to patients.
- Purity: 99.0%
- CAS No.: 10285-06-0
- Formula: C15H25NO5
- Molecular Weight:299.36
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) (+)-Intermedine
More
Biological Activity
(+)-Intermedine (1.1-30 μM; 24 hours) exhibits significant cytotoxicity at 30 μM and reduces cell viability in a concentration dependent manner in neural progenitor cells (NPCs)[1].
(+)-Intermedine showsno differentiation impairment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Neural progenitor cells (NPCs)
-
Concentration:1.1, 3.3, 10, 30 μM
-
Incubation Time:24 hours
-
Result:Exhibited significant cytotoxicity at 30 μM and reduced cell viability in a concentration dependent manner.
Chemical Information
-
CAS No. 10285-06-0
-
Appearance Solid
-
Molecular Weight 299.36
-
Formula C15H25NO5
-
Color White to off-white
-
SMILES
CC(C)[C@@]([C@H](O)C)(O)C(OCC1=CCN2[C@@]1([H])[C@H](O)CC2)=O
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
Solvent & Solubility
DMSO : 50 mg/mL (167.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (4.18 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3405 mL | 16.7023 mL | 33.4046 mL | 83.5115 mL |
| 5 mM | 0.6681 mL | 3.3405 mL | 6.6809 mL | 16.7023 mL | |
| 10 mM | 0.3340 mL | 1.6702 mL | 3.3405 mL | 8.3511 mL | |
| 15 mM | 0.2227 mL | 1.1135 mL | 2.2270 mL | 5.5674 mL | |
| 20 mM | 0.1670 mL | 0.8351 mL | 1.6702 mL | 4.1756 mL | |
| 25 mM | 0.1336 mL | 0.6681 mL | 1.3362 mL | 3.3405 mL | |
| 30 mM | 0.1113 mL | 0.5567 mL | 1.1135 mL | 2.7837 mL | |
| 40 mM | 0.0835 mL | 0.4176 mL | 0.8351 mL | 2.0878 mL | |
| 50 mM | 0.0668 mL | 0.3340 mL | 0.6681 mL | 1.6702 mL | |
| 60 mM | 0.0557 mL | 0.2784 mL | 0.5567 mL | 1.3919 mL | |
| 80 mM | 0.0418 mL | 0.2088 mL | 0.4176 mL | 1.0439 mL | |
| 100 mM | 0.0334 mL | 0.1670 mL | 0.3340 mL | 0.8351 mL |
Powered by Bioz