PP185
PP185 is a insulin receptor kinase substrate. PP185 undergoes rapid tyrosine phosphorylation, with activity dependent on divalent cations and inhibited by insulin receptor kinase domain antibodies. PP185 can be used for the research of neuroblastoma.
For research use only. We do not sell to patients.
- CAS No.: 851176-40-4
- Formula: C11H14ClNO2
- Molecular Weight:227.69
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PP185 (0.4-200 μM; 1 h) engages an increasing number of cysteine sites in HEK293T cell lysates as its concentration increases, with detectable liganding events observed at higher concentrations[1].
PP185 is phosphorylated in a rapid and dose-dependent manner induced by both Insulin (HY-P0035) and IGF-I in mouse neuroblastoma N18 cells, with maximal phosphorylation at 1 min and rapid dephosphorylation thereafter[2].
PP185 from rat H-35 hepatoma cells has tyrosine phosphorylation in a cell-free system inhibited by anti-IRK (a site-specific antibody targeting insulin receptor kinase)[3].
PP185 in rat H-35 hepatoma cells undergoes rapid tyrosine phosphorylation induced by insulin treatment[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 851176-40-4
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Molecular Weight 227.69
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Formula C11H14ClNO2
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SMILES
O=C(N(C)CC=1C=CC=C(OC)C1)CCl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Biggs GS,et al. Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics. Nat Commun. 2025 Jan 2;16(1):73. [Content Brief]
[2]. Shemer J, et al. Insulin and insulin-like growth factor-I stimulate a common endogenous phosphoprotein substrate (pp185) in intact neuroblastoma cells. J Biol Chem. 1987;262(32):15476-15482. [Content Brief]
[3]. Tashiro-Hashimoto Y, et al. Tyrosine phosphorylation of pp185 by insulin receptor kinase in a cell-free system. J Biol Chem. 1989;264(12):6879-6885. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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