PROTAC HBeAg degrader-1

PROTAC HBeAg degrader-1 (compound LH-3) (10 μM; 24 h) reduces the level of total non-secretory HiBiT-tagged HBeAg in HEK293T HiBiT-HBeAg-NS cells to less than 30% of that in the DMSO control group^[1].
PROTAC HBeAg degrader-1 (20 μM; 24 h) reduces the level of secreted HiBiT-tagged HBeAg in HEK293T HiBiT-HBeAg-S cells to < 7% of that in the DMSO control group, and decreases the level of intracellular HiBiT-tagged HBeAg to 46% of that in the DMSO control group^[1].
PROTAC HBeAg degrader-1 (10 μM; 24 h) reduces secreted and intracellular HiBiT-tagged HBeAg in HEK293T HiBiT-HBeAg-S and HEK293T VHL KO HiBiT-HBeAg-S cells, indicating that this degradation is largely independent of VHL^[1].
PROTAC HBeAg degrader-1 (10 μM; 24 h) reduces secreted HiBiT-tagged HBeAg to 8% of the DMSO control level and intracellular HiBiT-tagged HBeAg to 44% of the DMSO control level in HEK293T HiBiT-HBeAg-S cells, and this activity depends on the key motif in the VH032 domain^[1].
PROTAC HBeAg degrader-1 (10 μM; 24 h) reduces secreted HiBiT-tagged HBeAg to 9% of the DMSO control level and intracellular HiBiT-tagged HBeAg to 45% of the DMSO control level in HEK293T HiBiT-HBeAg-S cells^[1].
PROTAC HBeAg degrader-1 (10 μM; 6 days) reduces the level of HBcAg (p21) in HBV-expressing HepAD38 cells^[1].
PROTAC HBeAg degrader-1 (10 μM; 24 h) reduces the level of secreted HBeAg in wild-type HBV-transfected Huh7 cells to approximately 8% of that in untreated cells, and reduces the level of this protein in HBV^T109I mutant-transfected Huh7 cells to approximately 11.5% of that in untreated cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.