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  5. Protamine sulfate

Protamine sulfate, polycationic peptide and a antiheparin agent, could neutralize the anticoagulant action of heparin and enhances lipid-mediated gene transfer.

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Protamine sulfate Chemical Structure

Protamine sulfate Chemical Structure

CAS No. : 9009-65-8

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Protamine sulfate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Protamine sulfate

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Description

Protamine sulfate, polycationic peptide and a antiheparin agent, could neutralize the anticoagulant action of heparin and enhances lipid-mediated gene transfer[1][2][3].

In Vitro

Protamine sulfate has an inhibitory effect on thrombin in the conversion of fibrinogen to fibrin, and that this inhibition is concentration dependent, partial, and reversible[3].
Protamine sulfate is a 5-kDa cationic polypeptide derived from salmon sperm that can bind negatively charged unfractionated heparin (UFH). Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Protamine sulfate Related Antibodies
In Vivo

Protamine sulfate can be used in animal modeling to construct animal cystitis models.
Induction of cystitis model[5]
Background
The presence and activity of protamine sulfate may trigger the activation and inflammatory response of the host immune system. These immune responses can lead to the infiltration of inflammatory cells and the production of inflammatory mediators, such as cytokines and interleukins, which can lead to cystitis.
Specific Mmodeling Methods
Rat: Sprague–Dawley • female &bull
Administration: 10 mg/mL irrigation in the urinary bladder • once
Note
(1) 10 mg/ml protamine sulfate was injected into the rat bladder, and 2 mg/ml LPS was injected 30 minutes later
(2) 5 days later, the experimental animals were put to death under anesthesia, and the bladder was removed for observation.


Modeling Indicators
Physiological indicators: The turbidity, urine volume and the number of red blood cells in the urine of rats were detected.
Molecular changes: The levels of biomarkers IL-6, TNF-α and NF-κB in bladder tissues were detected.
Histological analysis: Hematoxylin and eosin staining were used to detect the number of mast cells in the bladder of rats.
Correlated Product(s): LPS (HY-D1056)
Opposite Product(s):

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Protamine sulfate]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (Need ultrasonic)

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 33.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.99%

COA (242 KB) RP-HPLC (206 KB)

Handling Instructions (2659 KB)
References
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Protamine sulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Protamine9009-65-8ThrombinfibrinUFHthrombinInhibitorinhibitorinhibit

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