Pseudouridine 5'-triphosphate (Synonyms: Pseudo-UTP)

Cat. No.: HY-141567: FAQs
Data Sheet Handling Instructions

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pseudouridine-5’-triphosphate (Pseudo-UTP) is one of the most commonly used modified nucleoside for the polymerase-mediated synthesis of RNA molecules. Compared with uridine-containing unmodified mRNAs, the application of pseudouridine-containing modified mRNAs exhibits better nuclease stability, immunogenicity, and translational properties.
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At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Pseudouridine 5'-triphosphate trisodium) usually boasts enhanced water solubility and stability.

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Pseudouridine 5'-triphosphate Chemical Structure

CAS No. : 1175-34-4

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Other In-stock Forms of Pseudouridine 5'-triphosphate:

Other Forms of Pseudouridine 5'-triphosphate:

Pseudouridine 5'-triphosphate trisodium In-stock

1 Publications Citing Use of MCE Pseudouridine 5'-triphosphate

•Cell Rep. 2023 May 23;42(6):112550. [Abstract]

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Description

Molecular Weight

484.14

Formula

C₉H₁₅N₂O₁₅P₃

CAS No.

1175-34-4

SMILES

O[C@H]1[C@@H](O)[C@H](C(C(N2)=O)=CNC2=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 250 mg/mL (516.38 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0655 mL	10.3276 mL	20.6552 mL
5 mM	0.4131 mL	2.0655 mL	4.1310 mL
10 mM	0.2066 mL	1.0328 mL	2.0655 mL

View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Shanmugasundaram M, Senthilvelan A, Kore AR. Gram-Scale Chemical Synthesis of Base-Modified Ribonucleoside-5'-O-Triphosphates. Curr Protoc Nucleic Acid Chem. 2016;67:13.15.1-13.15.10. [Content Brief]

[2]. Borchardt EK, Martinez NM, Gilbert WV. Regulation and Function of RNA Pseudouridylation in Human Cells. Annu Rev Genet. 2020;54:309-336. [Content Brief]

[3]. Anderson BR, Muramatsu H, Nallagatla SR, et al. Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation. Nucleic Acids Res. 2010;38(17):5884-5892. [Content Brief]

[4]. Morais P, Adachi H, Yu YT. The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines. Front Cell Dev Biol. 2021;9:789427. Pu [Content Brief]

[5]. Muthian Shanmugasundaram, et al. An Efficient Protection-Free One-Pot Chemical Synthesis of Modified Nucleoside-5'-Triphosphates. Nucleosides Nucleotides Nucleic Acids. 2016 Jul 2;35(7):356-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.0655 mL	10.3276 mL	20.6552 mL	51.6380 mL
	5 mM	0.4131 mL	2.0655 mL	4.1310 mL	10.3276 mL
	10 mM	0.2066 mL	1.0328 mL	2.0655 mL	5.1638 mL
	15 mM	0.1377 mL	0.6885 mL	1.3770 mL	3.4425 mL
	20 mM	0.1033 mL	0.5164 mL	1.0328 mL	2.5819 mL
	25 mM	0.0826 mL	0.4131 mL	0.8262 mL	2.0655 mL
	30 mM	0.0689 mL	0.3443 mL	0.6885 mL	1.7213 mL
	40 mM	0.0516 mL	0.2582 mL	0.5164 mL	1.2909 mL
	50 mM	0.0413 mL	0.2066 mL	0.4131 mL	1.0328 mL
	60 mM	0.0344 mL	0.1721 mL	0.3443 mL	0.8606 mL
	80 mM	0.0258 mL	0.1291 mL	0.2582 mL	0.6455 mL
	100 mM	0.0207 mL	0.1033 mL	0.2066 mL	0.5164 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pseudouridine 5'-triphosphate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pseudouridine 5'-triphosphate1175-34-4Pseudo-UTPDNA/RNA SynthesisPseudouridine 5'-triphosphate (trisodium)Pseudo-UTP (trisodium)Inhibitorinhibitorinhibit

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