PSMα3
Based on 1 Customer Validation
PSMα3 is an inhibitor of NF-κB p65 and p38 MAPK. PSMα3 forms membrane pores and binds to residues of human insulin B chain to inhibit insulin aggregation. PSMα3 forms α-type amyloid-like fibrils to exert cytotoxic effects, and acts as a functional amyloid virulence determinant of Staphylococcus aureus. PSMα3 is applicable to research related to spondyloarthritis, rheumatoid arthritis, insulin-derived amyloidosis, and Staphylococcus aureus infection.
For research use only. We do not sell to patients.
- Purity: 99.39%
- CAS No.: 1001405-52-2
- Formula: C128H192N28O30S
- Molecular Weight:2635.13
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PSMα3 (10 μM; 6-24 h) alters surface molecule expression in TLR4-stimulated human moDCs, enhancing early HLA-DR expression and reducing CD40 and CD80 expression at 6 and 24 h, respectively, while showing a trend to reduce PD-L1 upregulation at 24 h[1].
PSMα3 (10 μM; 6-24 h) significantly impairs pro- and anti-inflammatory cytokine secretion by TLR4-stimulated human moDCs, reducing TNF, IL-12, and IL-10 production at 6 and 24 h, respectively[1].
PSMα3 (10 μM; 1 h) reduces NF-κB and p38 phosphorylation in a non-significant trend in TLR4-stimulated human moDCs after 1 h of treatment[1].
PSMα3 (10 μM; 24 h) significantly reduces OVA uptake by immature human moDCs after 24 h of treatment, and shows a non-significant trend to further reduce OVA uptake in TLR2- or TLR4-stimulated moDCs[1].
PSMα3 (2.5-10 μM; 10 min) induces concentration-dependent transient pore formation in human moDCs after 10 min of treatment, as measured by LDH release, without reducing cell viability[1].
PSMα3 (10 μM; 24 h moDC pretreatment) in combination with TLR2/TLR4 ligands reduces the frequency of T-bet+ Th1 cells and IFN-γ secretion in co-cultured human naïve CD4+ T cells, while increasing IL-13 secretion when used to pretreat moDCs[1].
PSMα3 (10 μM; 1-2 d) significantly increases IDO production by TLR4-stimulated human moDCs after 1 and 2 d of treatment[1].
Synthetic PSMα3 (0.0625-10 mg/mL; up to 7 days) maintains a stable α-helical conformation in aqueous solution and does not form amyloid fibrils after incubation for up to 7 days at 37 °C, with only minor oligomer formation observed under specific non-standard pre-treatment and incubation conditions[2].
PSMα3 forms a unique cross-α amyloid-like fibril structure, solved at 1.45 Å resolution, with amphipathic α-helices stacked perpendicular to the fibril axis into tight self-associating sheets[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1001405-52-2
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Appearance Solid
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Molecular Weight 2635.13
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Formula C128H192N28O30S
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Color White to off-white
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Sequence
{f}-Met-Glu-Phe-Val-Ala-Lys-Leu-Phe-Lys-Phe-Phe-Lys-Asp-Leu-Leu-Gly-Lys-Phe-Leu-Gly-Asn-Asn
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Sequence Shortening
{f}-MEFVAKLFKFFKDLLGKFLGNN
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Structure Classification
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Initial Source
S. aureus
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (37.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Richardson JR, et al. Staphylococcus aureus PSM Peptides Modulate Human Monocyte-Derived Dendritic Cells to Prime Regulatory T Cells. Front Immunol. 2018 Nov 13;9:2603. [Content Brief]
[2]. Kalitnik A, et al. Cytotoxic Staphylococcus aureus PSMα3 inhibits the aggregation of human insulin in vitro. Phys Chem Chem Phys. 2024 May 29;26(21):15587-15599. [Content Brief]
[3]. Tayeb-Fligelman E, et al. The cytotoxic Staphylococcus aureus PSMα3 reveals a cross-α amyloid-like fibril. Science. 2017 Feb 24;355(6327):831-833. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.3795 mL | 1.8974 mL | 3.7949 mL | 9.4872 mL |
| 5 mM | 0.0759 mL | 0.3795 mL | 0.7590 mL | 1.8974 mL | |
| 10 mM | 0.0379 mL | 0.1897 mL | 0.3795 mL | 0.9487 mL | |
| 15 mM | 0.0253 mL | 0.1265 mL | 0.2530 mL | 0.6325 mL | |
| 20 mM | 0.0190 mL | 0.0949 mL | 0.1897 mL | 0.4744 mL | |
| 25 mM | 0.0152 mL | 0.0759 mL | 0.1518 mL | 0.3795 mL | |
| 30 mM | 0.0126 mL | 0.0632 mL | 0.1265 mL | 0.3162 mL |
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