1. Cell Cycle/DNA Damage
    Apoptosis
  2. Topoisomerase
    Apoptosis
  3. Pyrazoloacridine

Pyrazoloacridine (Synonyms: NSC 366140; PD 115934)

Cat. No.: HY-108969 Purity: >98.0%
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Pyrazoloacridine (NSC 366140), an intercalating agent with anti-cancer activity, inhibits the activity of topoisomerases 1 and 2. Pyrazoloacridine (NSC 366140) exhibits an IC50 of 1.25 μM in K562 myeloid leukemia cells for 24 h treatment.

For research use only. We do not sell to patients.

Pyrazoloacridine Chemical Structure

Pyrazoloacridine Chemical Structure

CAS No. : 99009-20-8

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Description

Pyrazoloacridine (NSC 366140), an intercalating agent with anti-cancer activity, inhibits the activity of topoisomerases 1 and 2. Pyrazoloacridine (NSC 366140) exhibits an IC50 of 1.25 μM in K562 myeloid leukemia cells for 24 h treatment[1][2].

In Vitro

Pyrazoloacridine (NSC 366140, PD 115934) exhibits IC50 values of 10.7 μM and 4.5 μM for oxic and hypoxic HCT-8 cells[1].
Pyrazoloacridine (NSC 366140, 2-4 μM) abolishes the catalytic activity of both topo I and topo II in vitro[2].
Pyrazoloacridine (NSC 366140) displays activity against cisplatin- and paclitaxel-resistant ovarian cancer[2].
Pyrazoloacridine (NSC 366140) has been shown to cause delayed DNA fragmentation in MCF-7 breast cancer cells[2].
Pyrazoloacridine (NSC 366140) induces apoptosis in P53-deficient Hep 3B human hepatoma cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[2]

Cell Line: K562 Myeloid Leukemia Cells.
Concentration: 0-500 μM.
Incubation Time: 1 h or 24 h.
Result: When K562 cells were incubated with PA for 1 h and then plated in soft agar, an IC50 of -50 μM was observed. In contrast, when cells were incubated for 24 h with PA, the IC50 was 1.25 μM.
Molecular Weight

367.40

Formula

C₁₉H₂₁N₅O₃

CAS No.

99009-20-8

SMILES

O=[N+](C1=CC=C2C3=C1NC4=CC=C(OC)C=C4C3=NN2CCCN(C)C)[O-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
References

Purity: >98.0%

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Keywords:

PyrazoloacridineNSC 366140 PD 115934NSC366140NSC-366140PD115934PD 115934PD-115934TopoisomeraseApoptosissolidtumormyeloidleukemiapancreaticcarcinomaInhibitorinhibitorinhibit

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Pyrazoloacridine
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