RAD51-IN-1
Based on 1 Customer Validation
RAD51-IN-1, a derivative of B02, is a potent inhibitor of RAD51. RAD51-IN-1 can be used for cancer research.
For research use only. We do not sell to patients.
- Purity: 99.18%
- CAS No.: 2101739-18-6
- Formula: C22H16ClN3O
- Molecular Weight:373.83
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Aging Compound Library
HY-L034
-
Anti-Breast Cancer Compound Library
HY-L074
-
Targeted Diversity Library
HY-L099
-
Heterocyclic Compound Library
HY-L138
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| BT-549 | IC50 |
6.4 μM
Compound: 17
|
Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human BT549 cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| HCC1937 | IC50 |
13.7 μM
Compound: 17
|
Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human HCC1937 cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| Hs-578T | IC50 |
4.2 μM
Compound: 17
|
Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human Hs578T cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| MCF-10A | IC50 |
48.3 μM
Compound: 17
|
Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| MDA-MB-231 | IC50 |
6.7 μM
Compound: 17
|
Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| MDA-MB-436 | IC50 |
4.1 μM
Compound: 17
|
Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human MDA-MB-436 cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| MDA-MB-468 | IC50 |
5.7 μM
Compound: 17
|
Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human MDA-MB-468 cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
| SUM-159-PT | IC50 |
5.5 μM
Compound: 17
|
Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay
Cytotoxicity against human SUM159PT cells assessed as decrease in cell viability after 120 hrs by MTS assay
|
[PMID: 28545975] |
RAD51 is a vital component of the homologous recombination DNA repair pathway and is overexpressed in drug-resistant cancers, including aggressive triple-negative breast cancer (TNBC)[1].RAD51-IN-1 (10 μM) decreases the ratio of RAD51 positive cells/cH2AX positive cells in MDA-MB-231 cell exposure to 6 Gy irradiation[1].RAD51-IN-1 (10 μM) significantly inhibits DNA damage induced RAD51 foci formation with 6 Gy irradiation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2101739-18-6
-
Appearance Solid
-
Molecular Weight 373.83
-
Formula C22H16ClN3O
-
Color Yellow to orange
-
SMILES
O=C1N(CC2=CC=C(Cl)C=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 62.5 mg/mL (167.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6750 mL | 13.3751 mL | 26.7501 mL | 66.8753 mL |
| 5 mM | 0.5350 mL | 2.6750 mL | 5.3500 mL | 13.3751 mL | |
| 10 mM | 0.2675 mL | 1.3375 mL | 2.6750 mL | 6.6875 mL | |
| 15 mM | 0.1783 mL | 0.8917 mL | 1.7833 mL | 4.4584 mL | |
| 20 mM | 0.1338 mL | 0.6688 mL | 1.3375 mL | 3.3438 mL | |
| 25 mM | 0.1070 mL | 0.5350 mL | 1.0700 mL | 2.6750 mL | |
| 30 mM | 0.0892 mL | 0.4458 mL | 0.8917 mL | 2.2292 mL | |
| 40 mM | 0.0669 mL | 0.3344 mL | 0.6688 mL | 1.6719 mL | |
| 50 mM | 0.0535 mL | 0.2675 mL | 0.5350 mL | 1.3375 mL | |
| 60 mM | 0.0446 mL | 0.2229 mL | 0.4458 mL | 1.1146 mL | |
| 80 mM | 0.0334 mL | 0.1672 mL | 0.3344 mL | 0.8359 mL | |
| 100 mM | 0.0268 mL | 0.1338 mL | 0.2675 mL | 0.6688 mL |
Powered by Bioz