S-Trityl-L-cysteine
Based on 3 publication(s) in Google Scholar
S-Trityl-L-cysteine (NSC 83265) is a selective and allosteric kinesin Eg5 inhibitor with an IC50 of 1 μM for the inhibition of basal ATPase activity and 140 nM for the microtubule-activated ATPase activity. S-Trityl-L-cysteine has antitumor activities.
For research use only. We do not sell to patients.
- Purity: 99.53%
- CAS No.: 2799-07-7
- Formula: C22H21NO2S
- Molecular Weight:363.47
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) S-Trityl-L-cysteine
MoreAll Kinesin Isoforms
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Biological Activity
|
Eg5 |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | GI50 |
1.26 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human 786-0 cells
Growth inhibition of human 786-0 cells
|
[PMID: 21855351] |
| A549 | GI50 |
5.01 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human A549 cells
Growth inhibition of human A549 cells
|
[PMID: 21855351] |
| ACHN | GI50 |
19.9 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human ACHN cells
Growth inhibition of human ACHN cells
|
[PMID: 21855351] |
| BXPC-3 | GI50 |
1563 nM
Compound: 1, STLC
|
Antiproliferative activity against human BxPC3 cells after 72 hrs by Alamar blue assay
Antiproliferative activity against human BxPC3 cells after 72 hrs by Alamar blue assay
|
[PMID: 22248262] |
| BXPC-3 | GI50 |
1563 nM
Compound: 4
|
Growth inhibition of human BxPC3 cells after 72 hrs by Alamar blue assay
Growth inhibition of human BxPC3 cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| BXPC-3 | GI50 |
1563 nM
Compound: 4, STLC
|
Cytotoxicity against human BxPC3 cells after 72 hrs by Alamar blue assay
Cytotoxicity against human BxPC3 cells after 72 hrs by Alamar blue assay
|
[PMID: 22749640] |
| CAKI-1 | GI50 |
15.8 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human Caki1 cells
Growth inhibition of human Caki1 cells
|
[PMID: 21855351] |
| CCRF-CEM | GI50 |
1.58 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human CCRF-CEM cells
Growth inhibition of human CCRF-CEM cells
|
[PMID: 21855351] |
| COLO 205 | GI50 |
3.16 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human COLO205 cells
Growth inhibition of human COLO205 cells
|
[PMID: 21855351] |
| EKVX | GI50 |
3.16 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human EKVX cells
Growth inhibition of human EKVX cells
|
[PMID: 21855351] |
| HCC 2998 | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HCC2998 cells
Growth inhibition of human HCC2998 cells
|
[PMID: 21855351] |
| HCT-116 | GI50 |
0.5 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HCT116 cells
Growth inhibition of human HCT116 cells
|
[PMID: 21855351] |
| HCT-116 | GI50 |
553 nM
Compound: 1, STLC
|
Antiproliferative activity against human HCT116 cells after 72 hrs by Alamar blue assay
Antiproliferative activity against human HCT116 cells after 72 hrs by Alamar blue assay
|
[PMID: 22248262] |
| HCT-116 | GI50 |
553 nM
Compound: 4
|
Growth inhibition of human HCT116 cells after 72 hrs by Alamar blue assay
Growth inhibition of human HCT116 cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| HCT-116 | GI50 |
553 nM
Compound: 4, STLC
|
Cytotoxicity against human HCT116 cells after 72 hrs by Alamar blue assay
Cytotoxicity against human HCT116 cells after 72 hrs by Alamar blue assay
|
[PMID: 22749640] |
| HCT-116 | IC50 |
910 nM
Compound: 3; STLC
|
Growth inhibition of human HCT116 cells after 72 hrs by MTS assay
Growth inhibition of human HCT116 cells after 72 hrs by MTS assay
|
[PMID: 26396688] |
| HCT-15 | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HCT15 cells
Growth inhibition of human HCT15 cells
|
[PMID: 21855351] |
| HeLa | EC50 |
0.7 μM
Compound: 1, STLC
|
Induction of mitotic arrest in human HeLa cells assessed as monoastral spindle formation after 24 hrs
Induction of mitotic arrest in human HeLa cells assessed as monoastral spindle formation after 24 hrs
|
[PMID: 18266314] |
| HeLa | EC50 |
1.7 μM
Compound: 1, STLC
|
Induction of mitotic arrest in human HeLa cells after 24 hrs
Induction of mitotic arrest in human HeLa cells after 24 hrs
|
[PMID: 18266314] |
| HeLa | EC50 |
1.72 μM
Compound: 1, STLC, NSC-83265
|
Inhibition of KSP in human HeLa cells assessed as bipolar spindle formation
Inhibition of KSP in human HeLa cells assessed as bipolar spindle formation
|
[PMID: 21855351] |
| HeLa | IC50 |
3.29 μM
Compound: 1
|
Cytotoxic activity against HeLa cells
Cytotoxic activity against HeLa cells
|
[PMID: 17524640] |
| HL-60(TB) | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HL-60(TB) cells
Growth inhibition of human HL-60(TB) cells
|
[PMID: 21855351] |
| HOP-62 | GI50 |
19.9 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HOP62 cells
Growth inhibition of human HOP62 cells
|
[PMID: 21855351] |
| HT-29 | GI50 |
3.98 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human HT-29 cells
Growth inhibition of human HT-29 cells
|
[PMID: 21855351] |
| IGROV-1 | GI50 |
3.98 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human IGROV1 cells
Growth inhibition of human IGROV1 cells
|
[PMID: 21855351] |
| K562 | GI50 |
1.58 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human K562 cells
Growth inhibition of human K562 cells
|
[PMID: 21855351] |
| K562 | GI50 |
1452 nM
Compound: 1, STLC
|
Antiproliferative activity against human K562 cells after 72 hrs by Alamar blue assay
Antiproliferative activity against human K562 cells after 72 hrs by Alamar blue assay
|
[PMID: 22248262] |
| K562 | GI50 |
1452 nM
Compound: 4, STLC
|
Cytotoxicity against human K562 cells after 72 hrs by Alamar blue assay
Cytotoxicity against human K562 cells after 72 hrs by Alamar blue assay
|
[PMID: 22749640] |
| K562 | GI50 |
1791 nM
Compound: 4
|
Growth inhibition of human K562 cells after 72 hrs by Alamar blue assay
Growth inhibition of human K562 cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| KM12 | GI50 |
5.01 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human KM12 cells
Growth inhibition of human KM12 cells
|
[PMID: 21855351] |
| LLC-PK1 | GI50 |
1.06 μM
Compound: 1, STLC
|
Growth inhibition of pig LLC-PK1 cells
Growth inhibition of pig LLC-PK1 cells
|
[PMID: 21344920] |
| LLC-PK1 | GI50 |
1.11 μM
Compound: 1, STLC
|
Growth inhibition of pig LLC-PK1 cells in presence of P-glycoprotein inhibitor LY335979
Growth inhibition of pig LLC-PK1 cells in presence of P-glycoprotein inhibitor LY335979
|
[PMID: 21344920] |
| LNCaP | GI50 |
811 nM
Compound: 4
|
Growth inhibition of human LNCAP cells after 72 hrs by Alamar blue assay
Growth inhibition of human LNCAP cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| LOX IMVI | GI50 |
6.31 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human LOXIMVI cells
Growth inhibition of human LOXIMVI cells
|
[PMID: 21855351] |
| M14 | GI50 |
0.398 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human M14 cells
Growth inhibition of human M14 cells
|
[PMID: 21855351] |
| Malme-3M | GI50 |
1.26 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human MALME-3M cells
Growth inhibition of human MALME-3M cells
|
[PMID: 21855351] |
| MDCK-II | GI50 |
0.58 μM
Compound: 1, STLC
|
Growth inhibition of dog MDCK2-MDR1 cells overexpressing human P-glycoprotein in presence of P-glycoprotein inhibitor LY335979
Growth inhibition of dog MDCK2-MDR1 cells overexpressing human P-glycoprotein in presence of P-glycoprotein inhibitor LY335979
|
[PMID: 21344920] |
| MDCK-II | GI50 |
0.86 μM
Compound: 1, STLC
|
Growth inhibition of dog MDCK2 cells
Growth inhibition of dog MDCK2 cells
|
[PMID: 21344920] |
| MDCK-II | GI50 |
0.89 μM
Compound: 1, STLC
|
Growth inhibition of dog MDCK2 cells in presence of P-glycoprotein inhibitor LY335979
Growth inhibition of dog MDCK2 cells in presence of P-glycoprotein inhibitor LY335979
|
[PMID: 21344920] |
| MDCK-II | GI50 |
2.3 μM
Compound: 1, STLC
|
Growth inhibition of dog MDCK2-MDR1 cells overexpressing human P-glycoprotein
Growth inhibition of dog MDCK2-MDR1 cells overexpressing human P-glycoprotein
|
[PMID: 21344920] |
| MOLT-4 | GI50 |
7.94 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human MOLT4 cells
Growth inhibition of human MOLT4 cells
|
[PMID: 21855351] |
| NCI-H1299 | GI50 |
1549 nM
Compound: 1, STLC
|
Antiproliferative activity against human NCI-H1299 cells after 72 hrs by Alamar blue assay
Antiproliferative activity against human NCI-H1299 cells after 72 hrs by Alamar blue assay
|
[PMID: 22248262] |
| NCI-H1299 | GI50 |
1549 nM
Compound: 4
|
Growth inhibition of human NCI-H1299 cells after 72 hrs by Alamar blue assay
Growth inhibition of human NCI-H1299 cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| NCI-H1299 | GI50 |
1549 nM
Compound: 4, STLC
|
Cytotoxicity against human NCI-H1299 cells after 72 hrs by Alamar blue assay
Cytotoxicity against human NCI-H1299 cells after 72 hrs by Alamar blue assay
|
[PMID: 22749640] |
| NCI-H226 | GI50 |
100 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human NCI-H226 cells
Growth inhibition of human NCI-H226 cells
|
[PMID: 21855351] |
| NCI-H23 | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human NCI-H23 cells
Growth inhibition of human NCI-H23 cells
|
[PMID: 21855351] |
| NCI-H322M | GI50 |
1.26 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human NCI-H322M cells
Growth inhibition of human NCI-H322M cells
|
[PMID: 21855351] |
| NCI-H522 | GI50 |
0.5 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human NCI-H522 cells
Growth inhibition of human NCI-H522 cells
|
[PMID: 21855351] |
| OVCAR-3 | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human OVCAR3 cells
Growth inhibition of human OVCAR3 cells
|
[PMID: 21855351] |
| OVCAR-4 | GI50 |
12.6 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human OVCAR4 cells
Growth inhibition of human OVCAR4 cells
|
[PMID: 21855351] |
| OVCAR-5 | GI50 |
100 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human OVCAR5 cells
Growth inhibition of human OVCAR5 cells
|
[PMID: 21855351] |
| OVCAR-8 | GI50 |
1.99 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human OVCAR8 cells
Growth inhibition of human OVCAR8 cells
|
[PMID: 21855351] |
| PC-3 | GI50 |
1371 nM
Compound: 4
|
Growth inhibition of human PC3 cells after 72 hrs by Alamar blue assay
Growth inhibition of human PC3 cells after 72 hrs by Alamar blue assay
|
[PMID: 23394180] |
| SF-268 | GI50 |
3.98 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SF268 cells
Growth inhibition of human SF268 cells
|
[PMID: 21855351] |
| SF-295 | GI50 |
0.5 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SF295 cells
Growth inhibition of human SF295 cells
|
[PMID: 21855351] |
| SF-539 | GI50 |
0.79 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SF539 cells
Growth inhibition of human SF539 cells
|
[PMID: 21855351] |
| SK-MEL-2 | GI50 |
1.5 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SK-MEL-2 cells
Growth inhibition of human SK-MEL-2 cells
|
[PMID: 21855351] |
| SK-MEL-28 | GI50 |
12.6 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SK-MEL-28 cells
Growth inhibition of human SK-MEL-28 cells
|
[PMID: 21855351] |
| SK-MEL-5 | GI50 |
0.63 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SK-MEL-5 cells
Growth inhibition of human SK-MEL-5 cells
|
[PMID: 21855351] |
| SK-OV-3 | GI50 |
100 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SKOV3 cells
Growth inhibition of human SKOV3 cells
|
[PMID: 21855351] |
| SN12C | GI50 |
3.16 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SN12C cells
Growth inhibition of human SN12C cells
|
[PMID: 21855351] |
| SNB-19 | GI50 |
2.51 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SNB19 cells
Growth inhibition of human SNB19 cells
|
[PMID: 21855351] |
| SNB-75 | GI50 |
1.99 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SNB75 cells
Growth inhibition of human SNB75 cells
|
[PMID: 21855351] |
| SR | GI50 |
6.31 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SR cells
Growth inhibition of human SR cells
|
[PMID: 21855351] |
| SW-620 | GI50 |
1.58 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human SW620 cells
Growth inhibition of human SW620 cells
|
[PMID: 21855351] |
| TK-10 | GI50 |
6.31 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human TK10 cells
Growth inhibition of human TK10 cells
|
[PMID: 21855351] |
| U-251 | GI50 |
1 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human U251 cells
Growth inhibition of human U251 cells
|
[PMID: 21855351] |
| UACC-257 | GI50 |
1.26 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human UACC257 cells
Growth inhibition of human UACC257 cells
|
[PMID: 21855351] |
| UACC-62 | GI50 |
1.99 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human UACC62 cells
Growth inhibition of human UACC62 cells
|
[PMID: 21855351] |
| UO-31 | GI50 |
100 μM
Compound: 1, STLC, NSC-83265
|
Growth inhibition of human UO31 cells
Growth inhibition of human UO31 cells
|
[PMID: 21855351] |
S-Trityl-L-cysteine binds to a unique pocket in the Eg5 motor domain formed by secondary structural elements (helix a2/loop L5/helix a3)[1].
?
S-Trityl-L-cysteine (1-20 μM; for 72 h) could mediate cell apoptosis, as well as cell cycle arrest, in a dose-dependent manner. S-Trityl-L-cysteine-mediated apoptosis and cell cycle arrest were triggered by activation of the mitogen-activated protein kinase and nuclear factor kB signaling pathways[1].
?
S-Trityl-L-cysteine induces mitotic arrest in HeLa cells (IC50 of 700 nM) with characteristic monoastral spindles[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2799-07-7
-
Appearance Solid
-
Molecular Weight 363.47
-
Formula C22H21NO2S
-
Color Off-white to light yellow
-
SMILES
N[C@@H](CSC(C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3)C(O)=O
-
Synonyms
NSC 83265; S-Tritylcysteine; 3-Tritylthio-L-alanine
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
-
Journal Impact Factor
-
Most Recent
-
Cell Mol Immunol
2025 Nov 25. PMID: 41286079 -
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Solvent & Solubility
DMSO : 25 mg/mL (68.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.75 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.75 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wei Wu, et al. S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncol Lett. 2018 Jul;16(1):1023-1030. [Content Brief]
[2]. Salvatore DeBonis, et al. In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumor activities. Mol Cancer Ther. 2004 Sep;3(9):1079-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7513 mL | 13.7563 mL | 27.5126 mL | 68.7815 mL |
| 5 mM | 0.5503 mL | 2.7513 mL | 5.5025 mL | 13.7563 mL | |
| 10 mM | 0.2751 mL | 1.3756 mL | 2.7513 mL | 6.8781 mL | |
| 15 mM | 0.1834 mL | 0.9171 mL | 1.8342 mL | 4.5854 mL | |
| 20 mM | 0.1376 mL | 0.6878 mL | 1.3756 mL | 3.4391 mL | |
| 25 mM | 0.1101 mL | 0.5503 mL | 1.1005 mL | 2.7513 mL | |
| 30 mM | 0.0917 mL | 0.4585 mL | 0.9171 mL | 2.2927 mL | |
| 40 mM | 0.0688 mL | 0.3439 mL | 0.6878 mL | 1.7195 mL | |
| 50 mM | 0.0550 mL | 0.2751 mL | 0.5503 mL | 1.3756 mL | |
| 60 mM | 0.0459 mL | 0.2293 mL | 0.4585 mL | 1.1464 mL |