2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. SMases D-IN-1

SMases D-IN-1 is an inhibitor of SMase D (sphingomyelinase D) from Loxosceles (brown recluse spider), with a Ki value of 0.54 μM. SMases D-IN-1 inhibits the hydrolysis of sphingomyelin substrates by recombinant and native SMases D, reduces the binding ability of SMases D to human red blood cells, and prevents the shedding of glycophorin C from the surface of human red blood cells. SMases D-IN-1 partially inhibits Loxosceles venom-induced death of human keratinocytes and also suppresses systemic reactions triggered by Loxosceles venom. SMases D-IN-1 can be used in studies related to recluse spider envenomation.

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SMases D-IN-1

SMases D-IN-1 Chemical Structure

CAS No. : 849020-48-0

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SMases D-IN-1 Related Antibodies

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Description

SMases D-IN-1 is an inhibitor of SMase D (sphingomyelinase D) from Loxosceles (brown recluse spider), with a Ki value of 0.54 μM. SMases D-IN-1 inhibits the hydrolysis of sphingomyelin substrates by recombinant and native SMases D, reduces the binding ability of SMases D to human red blood cells, and prevents the shedding of glycophorin C from the surface of human red blood cells. SMases D-IN-1 partially inhibits Loxosceles venom-induced death of human keratinocytes and also suppresses systemic reactions triggered by Loxosceles venom. SMases D-IN-1 can be used in studies related to recluse spider envenomation[1].

In Vitro

SMases D-IN-1 (Compound 1) (20 μg; 30 min at 37 °C) reduces SMase D binding to human erythrocytes and completely prevents venom-induced glycophorin C cleavage from human erythrocyte surfaces[1].
SMases D-IN-1 (5 μg; 72 h) partially reduces L. laeta venom-induced cell death in human HaCaT keratinocytes[1].
SMases D-IN-1 (5 μg; 72 h) normalizes MMP-2 and MMP-9 secretion in human HaCaT keratinocytes exposed to L. laeta venom[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SMases D-IN-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: human HaCaT keratinocytes
Concentration: 5 μg
Incubation Time: 72 h
Result: Partially prevented L.
laeta venom-induced HaCaT cell death.
In Vivo

SMases D-IN-1 (Compound 1) (1 mg; i.d.; single dose) does not significantly reduce Loxosceles laeta venom-induced dermonecrosis or associated histopathological damage in albino New Zealand rabbits[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Albino New Zealand (male, 3 kg)[1]
Dosage: 1 mg
Administration: i.d.; single dose
Result: Had no significant effect on the development of venom-induced dermonecrotic lesions over 72 hours.
Showed persistent haemorrhagic areas, dense inflammatory infiltrate, collagen disorganisation, and adjacent muscle layer injury in skin samples, similar to venom-only controls.
Molecular Weight

412.49

Formula

C19H16N4O3S2
CAS No.
SMILES

N#CC1=CC=C(CSC2=NC(N)=CC(OS(=O)(C3=CC=C(C)C=C3)=O)=N2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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SMases D-IN-1 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMases D-IN-1849020-48-0Phospholipaseglycophorin Chuman keratinocyteloxoscelismsphingomyelinhuman erythrocytesalbino New Zealand rabbitsLoxosceles sphingomyelinase DLoxosceles venomhuman HaCaT keratinocytesLoxosceles laeta SMase DInhibitorinhibitorinhibit

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