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Cat. No.: HY-182413

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SMases D-IN-1

SMases D-IN-1 is an inhibitor of SMase D (sphingomyelinase D) from Loxosceles (brown recluse spider), with a K_i value of 0.54 μM. SMases D-IN-1 inhibits the hydrolysis of sphingomyelin substrates by recombinant and native SMases D, reduces the binding ability of SMases D to human red blood cells, and prevents the shedding of glycophorin C from the surface of human red blood cells. SMases D-IN-1 partially inhibits Loxosceles venom-induced death of human keratinocytes and also suppresses systemic reactions triggered by Loxosceles venom. SMases D-IN-1 can be used in studies related to recluse spider envenomation.

For research use only. We do not sell to patients.

CAS No.: 849020-48-0
Formula: C₁₉H₁₆N₄O₃S₂
Molecular Weight:412.49

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

SMases D-IN-1 Chemical Structure

CAS No.: 849020-48-0

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
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Biological Activity

Description

All Phospholipase Isoforms :

In Vitro

SMases D-IN-1 (Compound 1) (20 μg; 30 min at 37 °C) reduces SMase D binding to human erythrocytes and completely prevents venom-induced glycophorin C cleavage from human erythrocyte surfaces^[1].
SMases D-IN-1 (5 μg; 72 h) partially reduces L. laeta venom-induced cell death in human HaCaT keratinocytes^[1].
SMases D-IN-1 (5 μg; 72 h) normalizes MMP-2 and MMP-9 secretion in human HaCaT keratinocytes exposed to L. laeta venom^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

human HaCaT keratinocytes
Concentration:

5 μg
Incubation Time:

72 h
Result:

Partially prevented L.
laeta venom-induced HaCaT cell death.

In Vivo

SMases D-IN-1 (Compound 1) (1 mg; i.d.; single dose) does not significantly reduce Loxosceles laeta venom-induced dermonecrosis or associated histopathological damage in albino New Zealand rabbits^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Albino New Zealand (male, 3 kg)^[1]
Dosage:

1 mg
Administration:

i.d.; single dose
Result:

Had no significant effect on the development of venom-induced dermonecrotic lesions over 72 hours.
Showed persistent haemorrhagic areas, dense inflammatory infiltrate, collagen disorganisation, and adjacent muscle layer injury in skin samples, similar to venom-only controls.

Chemical Information

CAS No. 849020-48-0
Molecular Weight 412.49
Formula C₁₉H₁₆N₄O₃S₂
SMILES

N#CC1=CC=C(CSC2=NC(N)=CC(OS(=O)(C3=CC=C(C)C=C3)=O)=N2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Lopes PH, et al. Targeting Loxosceles spider Sphingomyelinase D with small-molecule inhibitors as a potential therapeutic approach for loxoscelism. J Enzyme Inhib Med Chem. 2019;34(1):310-321. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References

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SMases D-IN-1

All Phospholipase Isoforms