2. Membrane Transporter/Ion Channel
  3. CFTR
  4. SRI-41315

SRI-41315 induces a prolonged pause at stop codons and suppresses PTCs (premature termination codons) associated with cystic fibrosis in immortalized and primary human bronchial epithelial cells, restoring CFTR (cystic fibrosis transmembrane conductance regulator) expression and function. SRI-41315 suppresses PTCs by reducing the abundance of the termination factor eRF1. SRI-41315 also potentiates aminoglycoside-mediated readthrough, leading to synergistic increases in CFTR activity.

For research use only. We do not sell to patients.

CAS No. : 1613509-49-1

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Based on 1 publication(s) in Google Scholar

SRI-41315 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SRI-41315

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    SRI-41315 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Apr 5;15(1):2957.  [Abstract]

    Sup-tRNA mediated readthrough efficacy in CFBE41o- cells, with and without eRF1 inhibitor (SRI-41315, 5 µM) and normalized to wildtype FLuc extended with the same sequence without the PTC (eFLuc).

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    Description

    SRI-41315 induces a prolonged pause at stop codons and suppresses PTCs (premature termination codons) associated with cystic fibrosis in immortalized and primary human bronchial epithelial cells, restoring CFTR (cystic fibrosis transmembrane conductance regulator) expression and function. SRI-41315 suppresses PTCs by reducing the abundance of the termination factor eRF1. SRI-41315 also potentiates aminoglycoside-mediated readthrough, leading to synergistic increases in CFTR activity[1].

    In Vitro

    SRI-41315 exhibits target cell cytotoxicity (CC50) values >50 μM in both FRT and 16BE14o- cells[1].
    SRI-41315 shows improved potency and efficacy in FRT cells that translated to 16HBE14o- cells[1].
    SRI-41315 (5 μM, 20 h) depletes eRF1 levels through a proteasome-mediated degradation pathway[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SRI-41315 Related Antibodies

    Western Blot Analysis[1]

    Cell Line: CFTR-G542X 16HBEge G542X cells
    Concentration: 5 µM
    Incubation Time: 20 h
    Result: Depleted eRF1 levels through a proteasome-mediated degradation pathway. SRI-41315-mediated eRF1 degradation was prevented by the addition of (S)-MG132 but not the neddylation inhibitor MLN4924.
    Molecular Weight

    357.41

    Formula

    C22H19N3O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C2=C(N(C)C(N=C(C3CCC3)N4C5=CC=CC=C5)=C1C4=O)C=CC=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 20.83 mg/mL (58.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7979 mL 13.9895 mL 27.9791 mL
    5 mM 0.5596 mL 2.7979 mL 5.5958 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7979 mL 13.9895 mL 27.9791 mL 69.9477 mL
    5 mM 0.5596 mL 2.7979 mL 5.5958 mL 13.9895 mL
    10 mM 0.2798 mL 1.3990 mL 2.7979 mL 6.9948 mL
    15 mM 0.1865 mL 0.9326 mL 1.8653 mL 4.6632 mL
    20 mM 0.1399 mL 0.6995 mL 1.3990 mL 3.4974 mL
    25 mM 0.1119 mL 0.5596 mL 1.1192 mL 2.7979 mL
    30 mM 0.0933 mL 0.4663 mL 0.9326 mL 2.3316 mL
    40 mM 0.0699 mL 0.3497 mL 0.6995 mL 1.7487 mL
    50 mM 0.0560 mL 0.2798 mL 0.5596 mL 1.3990 mL
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    SRI-41315 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SRI-413151613509-49-1SRI41315SRI 41315CFTRCystic fibrosis transmembrane conductance regulatorRNARNA decayCystic fibrosisPremature termination codonsPTCsFRT cellsInhibitorinhibitorinhibit

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