1. Membrane Transporter/Ion Channel
  2. CFTR
  3. SRI-41315

SRI-41315 

Cat. No.: HY-150090
Handling Instructions

SRI-41315 induces a prolonged pause at stop codons and suppresses PTCs (premature termination codons) associated with cystic fibrosis in immortalized and primary human bronchial epithelial cells, restoring CFTR (cystic fibrosis transmembrane conductance regulator) expression and function. SRI-41315 suppresses PTCs by reducing the abundance of the termination factor eRF1. SRI-41315 also potentiates aminoglycoside-mediated readthrough, leading to synergistic increases in CFTR activity.

For research use only. We do not sell to patients.

SRI-41315 Chemical Structure

SRI-41315 Chemical Structure

CAS No. : 1613509-49-1

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Description

SRI-41315 induces a prolonged pause at stop codons and suppresses PTCs (premature termination codons) associated with cystic fibrosis in immortalized and primary human bronchial epithelial cells, restoring CFTR (cystic fibrosis transmembrane conductance regulator) expression and function. SRI-41315 suppresses PTCs by reducing the abundance of the termination factor eRF1. SRI-41315 also potentiates aminoglycoside-mediated readthrough, leading to synergistic increases in CFTR activity[1].

In Vitro

SRI-41315 exhibits target cell cytotoxicity (CC50) values >50 µM in both FRT and 16BE14o- cells[1].
SRI-41315 shows improved potency and efficacy in FRT cells that translated to 16HBE14o- cells[1].
SRI-41315 (5 µM, 20 h) depletes eRF1 levels through a proteasome-mediated degradation pathway[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CFTR-G542X 16HBEge G542X cells
Concentration: 5 µM
Incubation Time: 20 h
Result: Depleted eRF1 levels through a proteasome-mediated degradation pathway. SRI-41315-mediated eRF1 degradation was prevented by the addition of (S)-MG132 but not the neddylation inhibitor MLN4924.
Molecular Weight

357.41

Formula

C22H19N3O2

CAS No.
SMILES

O=C1C2=C(N(C)C(N=C(C3CCC3)N4C5=CC=CC=C5)=C1C4=O)C=CC=C2

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Please store the product under the recommended conditions in the Certificate of Analysis.

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SRI-41315
Cat. No.:
HY-150090
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