country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-188367

Copy Link Print Information

Steviol glucuronide

Steviol glucuronide is a metabolite of Steviol (HY-N2057). Steviol glucuronide upregulates Glucagon receptor transcription and downregulates β-2-microglobulin transcription. Steviol glucuronide scavenges hydroxyl/superoxide radicals and reduces thiobarbituric acid reactive substances, without DPPH/nitric oxide scavenging activity. Steviol glucuronide stimulates glucose-dependent insulin secretion and does not affect basal insulin under low-glucose conditions. Steviol glucuronide enters cells via passive diffusion and OAT3/OATP1B1/OATP1B3/OATP2B1-mediated uptake, and inhibits OATP1B1/OATP1B3-mediated transport of Rosuvastatin (HY-17504B). Steviol glucuronide can be used in research on type 2 diabetes.

For research use only. We do not sell to patients.

CAS No.: 885044-59-7
Formula: C₂₆H₃₈O₉
Molecular Weight:494.57

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (286 KB)

Steviol glucuronide Chemical Structure

CAS No.: 885044-59-7

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC50	26.4 μM	Inhibition of OATP1B1-mediated uptake of 1 μM rosuvastatin in HEK293 cells after 30 min preincubation at 37°C measured by LC-MS/MS. Inhibition of OATP1B1-mediated uptake of 1 μM rosuvastatin in HEK293 cells after 30 min preincubation at 37°C measured by LC-MS/MS.	26525112
HEK293	IC50	39.8 μM	Inhibition of OATP1B3-mediated uptake of 1 μM rosuvastatin in HEK293 cells after 30 min preincubation at 37°C measured by LC-MS/MS. Inhibition of OATP1B3-mediated uptake of 1 μM rosuvastatin in HEK293 cells after 30 min preincubation at 37°C measured by LC-MS/MS.	26525112

In Vitro

Carbohydrate MetabolismOxidative StressOthers

Carbohydrate Metabolism

Steviol glucuronide (0.01 nM-10 μM; 1 h) stimulates insulin secretion from isolated mouse islets in a dose- and glucose-dependent manner, with the maximal enhancing effect at 0.1 μM in the presence of 16.7 mM glucose^[1].
Steviol glucuronide (0.1 μM; 72 h) alters the transcription of B2m and Gcgr in isolated mouse pancreatic islets after 72 h and produces numerical increases in Pdk1, Nkx2-2, Pdx1, Nkx6.1, Akt1, and Insr^[1].

Oxidative Stress

Steviol glucuronide (approximately 16 h) scavenges hydroxyl radicals in the cell-free TPA/HTPA assay with an IC₅₀ of 0.206 mM^[2].
Steviol glucuronide scavenges superoxide radicals in the cell-free NBT/NADH/PMS assay, with an IC₅₀ of 0.211 mM^[2].
Steviol glucuronide (30 min) shows no DPPH radical scavenging activity in the cell-free DPPH assay^[2].
Steviol glucuronide (25°C, protected from light for 150 min) shows no NO free radical scavenging activity in the cell-free sodium nitroprusside/Griess assay^[2].

Others

Steviol glucuronide (5-30 µM; 5 min) uptake in HEK293 cells is primarily mediated by OAT3 rather than OATP1B1, OATP1B3, and OATP2B1^[4].
Steviol glucuronide has an uptake K_m of 143.3 µM in HEK293-OAT3 cells and exhibits the highest intrinsic clearance (2.64 µl/min/mg protein) among the uptake transporters tested^[4].
Steviol glucuronide (0-300 µM; 30 min) inhibits the uptake of Rosuvastatin (HY-17504B) by HEK293-OATP1B1 and -OATP1B3 cells, with IC₅₀ values of 26.4 and 39.8 µM, respectively, and weakly inhibits OAT3-mediated uptake^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

mouse pancreatic islets
Concentration:

0.1 μM
Incubation Time:

72 h
Result:

Significantly decreased transcript level of beta‐2‐microglobulin (B2m) and significantly increased transcript level of glucagon receptor by 102% (Gcgr).
Upregulated transcript level of pyruvate dehydrogenase kinase, isoenzyme 1 (Pdk1), nearly reached a significant level.

Related Protocols :

Chemical Information

CAS No. 885044-59-7
Molecular Weight 494.57
Formula C₂₆H₃₈O₉
SMILES

[H][C@@]12CC[C@](O)(C3)C(C[C@]31CC[C@@]4([C@](C)(C(O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)C(O)=O)O)O)O)=O)CCC[C@]42C)[H])=C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gu W, et al. Steviol glucuronide, a metabolite of steviol glycosides, potently stimulates insulin secretion from isolated mouse islets: Studies in vitro. Endocrinology, diabetes & metabolism. 2019 Oct;2(4):e00093.

[2]. Hajihashemi S, et al. Free radical scavenging activity of steviol glycosides, steviol glucuronide, hydroxytyrosol, metformin, aspirin and leaf extract of Stevia rebaudiana[J]. Free Radicals and Antioxidants, 2013, 3: S34-S41.

[3]. Geuns JM, et al. Identification of steviol glucuronide in human urine. Journal of agricultural and food chemistry. 2006 Apr 05;54(7):2794-8.

[4]. Wang M, et al. Transmembrane transport of steviol glucuronide and its potential interaction with selected drugs and natural compounds. Food Chem Toxicol. 2015 Dec;86:217-24.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote

Amount: USD 0.00

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Steviol glucuronide