2. Cell Cycle/DNA Damage Stem Cell/Wnt
  3. Casein Kinase
  4. TBCA

TBCA is a highly selective CK2 (casein kinase II) inhibitor with an IC50 of 110 nM and a Ki of 77 nM. TBCA shows selectivity for CK2 over CK1, DYRK1A and a panel of 27 other kinases.

For research use only. We do not sell to patients.

TBCA Chemical Structure

TBCA Chemical Structure

CAS No. : 934358-00-6

Size Price Stock Quantity
5 mg USD 130 In-stock
10 mg USD 210 In-stock
25 mg USD 483 In-stock
50 mg USD 724 In-stock
100 mg USD 1086 In-stock
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500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

TBCA Related Antibodies

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  • Purity & Documentation

  • References

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Description

TBCA is a highly selective CK2 (casein kinase II) inhibitor with an IC50 of 110 nM and a Ki of 77 nM. TBCA shows selectivity for CK2 over CK1, DYRK1A and a panel of 27 other kinases[1][2].

IC50 & Target[1]

CK2

110 nM (IC50)

CK2

77 nM (Ki)

In Vitro

TBCA (0-100 μM; 24 hours) shows a drastic effect on Jurkat cells viability[1].
TBCA also inhibits CK2 double mutant (Val66 Ile174) with an IC50 of 0.68 μM[1].
TBCA dose-dependently inhibits platelet aggregation and secretion induced by various agonists including 2-MeSADP, AYPGKF, SFLLRN, and CRP. TBCA also inhibits phosphorylation of PDK1, Akt, and GSK3β induced by AYPGKF[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TBCA Related Antibodies

Cell Viability Assay[1]

Cell Line: Jurkat cells
Concentration: 0-100 μM
Incubation Time: 24 hours
Result: The viability of Jurkat cells was reduced.
Molecular Weight

463.74

Formula

C9H4Br4O2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

OC(/C=C/C1=C(C(Br)=C(Br)C(Br)=C1)Br)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (215.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1564 mL 10.7819 mL 21.5638 mL
5 mM 0.4313 mL 2.1564 mL 4.3128 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (5.39 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.60%

COA (247 KB) HNMR (141 KB) LCMS (252 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1564 mL 10.7819 mL 21.5638 mL 53.9095 mL
5 mM 0.4313 mL 2.1564 mL 4.3128 mL 10.7819 mL
10 mM 0.2156 mL 1.0782 mL 2.1564 mL 5.3910 mL
15 mM 0.1438 mL 0.7188 mL 1.4376 mL 3.5940 mL
20 mM 0.1078 mL 0.5391 mL 1.0782 mL 2.6955 mL
25 mM 0.0863 mL 0.4313 mL 0.8626 mL 2.1564 mL
30 mM 0.0719 mL 0.3594 mL 0.7188 mL 1.7970 mL
40 mM 0.0539 mL 0.2695 mL 0.5391 mL 1.3477 mL
50 mM 0.0431 mL 0.2156 mL 0.4313 mL 1.0782 mL
60 mM 0.0359 mL 0.1797 mL 0.3594 mL 0.8985 mL
80 mM 0.0270 mL 0.1348 mL 0.2695 mL 0.6739 mL
100 mM 0.0216 mL 0.1078 mL 0.2156 mL 0.5391 mL
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TBCA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TBCA934358-00-6Casein KinaseJurkatcellsviabilityselectivityplateletaggregationInhibitorinhibitorinhibit

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