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Cat. No.: HY-145859

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Topo I-IN-1

Topo I-IN-1 (Compound 14d) is a potent Topo I inhibitor with antitumor activity and DNA intercalative capability. Topo I-IN-1 induces cell apoptosis.

For research use only. We do not sell to patients.

CAS No.: 2763655-68-9
Formula: C₂₀H₁₄BrN₃O₂
Molecular Weight:408.25

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

Topo I-IN-1 Chemical Structure

CAS No.: 2763655-68-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Topo I-IN-1 (Compound 14d) is a potent Topo I inhibitor with antitumor activity and DNA intercalative capability. Topo I-IN-1 induces cell apoptosis^[1].

IC₅₀ & Target

Topoisomerase I

All Topoisomerase Isoforms :

In Vitro

Topo I-IN-1 (Compound 14d) (0-50 µM, 48 h) shows antiproliferative activity against human cancer cells and has low toxicity toward normal rat kidney epithelial cells^[1].
Topo I-IN-1 (0-4 µM, 48 h) shows a dose-dependent apoptotic effect, induces ROS generation, and inhibits cell migration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

A549, HCT116, MCF7, SK-MEL-28, and NRK (normal rat kidney epithelial cells)
Concentration:

0-50 µM
Incubation Time:

48 h
Result:

Showed in vitro cytotoxicity with IC₅₀ values of 2.33 ± 1.52, 2.85 ± 0.83, 26.23 ± 2.48, 3.86 ± 0.33, and 29.99 ± 0.95 µM against A549, HCT116, MCF7, SK-MEL-28, and NRK cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

A549
Concentration:

2, 2.33 and 4 μM
Incubation Time:

48 h
Result:

Resulted in the morphological changes typical to apoptosis.

Chemical Information

CAS No. 2763655-68-9
Molecular Weight 408.25
Formula C₂₀H₁₄BrN₃O₂
SMILES

O=C1NCC/C(C2=C1NC3=C2C=CC=C3)=C4C(NC5=C/4C=CC(Br)=C5)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Manasa Kadagathur, et al. Synthesis of indolo/pyrroloazepinone-oxindoles as potential cytotoxic, DNA-intercalating and Topo I inhibitors. Bioorg Chem. 2022 May;122:105706. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Topo I-IN-1

All Topoisomerase Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Topo I-IN-1 Related Classifications

HY-145859 Related Classifications